通过16S rDNA PCR-RFLP快速鉴别中间普氏菌和变黑普氏菌。

Rapid differentiation of Prevotella intermedia and P. nigrescens by 16S rDNA PCR-RFLP.

作者信息

Milsom S E, Sprague S V, Dymock D, Weightman A J, Wade W G

机构信息

Department of Oral and Dental Science, University of Bristol.

出版信息

J Med Microbiol. 1996 Jan;44(1):41-3. doi: 10.1099/00222615-44-1-41.

DOI:10.1099/00222615-44-1-41

PMID:8544210

Abstract

Prevotella intermedia and the newly described P. nigrescens cannot be reliably distinguished by phenotypic tests. In this study, restriction endonuclease digestion of amplified 16S rDNA (16S rDNA PCR-RFLP) was used to generate restriction profiles of the type strains of P. intermedia and P. nigrescens and 43 fresh isolates identified as belonging to one of the two species. Whole-cell protein profiles were obtained by SDS-PAGE for comparative purposes. The type strains of P. intermedia and P. nigrescens were easily distinguished by 16S rDNA PCR-RFLP and the fresh isolates were assigned to either species on the basis of their restriction profiles. The identifications obtained were identical to those obtained by protein profiles. 16S rDNA PCR-RFLP is a rapid and reliable method for the differentiation of P. intermedia and P. nigrescens.

摘要

中间普氏菌和新描述的变黑普氏菌无法通过表型试验可靠地区分。在本研究中，扩增的16S rDNA的限制性内切酶消化（16S rDNA PCR-RFLP）被用于生成中间普氏菌和变黑普氏菌模式菌株以及43株被鉴定属于这两个物种之一的新鲜分离株的限制性图谱。为了进行比较，通过SDS-PAGE获得全细胞蛋白质图谱。中间普氏菌和变黑普氏菌的模式菌株很容易通过16S rDNA PCR-RFLP区分，新鲜分离株根据其限制性图谱被归为这两个物种中的一个。所获得的鉴定结果与通过蛋白质图谱获得的结果一致。16S rDNA PCR-RFLP是区分中间普氏菌和变黑普氏菌的一种快速且可靠的方法。