Lee J H, Graybosch R A, Kaeppler S M, Sears R G
Department of Agronomy, University of Nebraska, Lincoln 68583, USA.
Genome. 1996 Jun;39(3):605-8. doi: 10.1139/g96-076.
A 2RL.2BS wheat-rye translocation, present in the wheat germplasm line Hamlet, carries a gene for resistance to Hessian fly biotype L, one of the most virulent biotypes presently encountered in wheat production environments. Unlike several other wheat-rye chromosome translocations common in wheat breeding programs, 2RL lacks genes encoding storage proteins or other easily selected markers. Oligonucleotide primers synthesized from published sequences derived from the R173 family of moderately repetitive rye DNA were used in the DNA polymerase chain reaction (PCR) to identify specific markers for 2RL. The same primers, when used with DNA extracted from additional wheat-rye translocation lines of importance to the wheat breeding community, gave distinctive PCR products for each genotype. The single primer pair, PAWS5 and PAWS6, may, therefore, have wide applicability for the identification of wheat-rye chromosomal translocations presently encountered in wheat breeding populations.
存在于小麦种质系哈姆雷特中的一种2RL.2BS小麦-黑麦易位系，携带有对黑森瘿蚊生物型L的抗性基因，生物型L是目前在小麦生产环境中遇到的毒性最强的生物型之一。与小麦育种计划中常见的其他几种小麦-黑麦染色体易位不同，2RL缺乏编码贮藏蛋白或其他易于选择的标记的基因。从来源于中度重复黑麦DNA的R173家族的已发表序列合成的寡核苷酸引物用于DNA聚合酶链反应（PCR），以鉴定2RL的特异性标记。当将相同的引物与从小麦育种界重要的其他小麦-黑麦易位系中提取的DNA一起使用时，每种基因型都产生了独特的PCR产物。因此，单一引物对PAWS5和PAWS6可能广泛适用于鉴定目前在小麦育种群体中遇到的小麦-黑麦染色体易位。
