Klemsdal S S, Hayes C K, Hjeljord L, Lorito M, Harman G E, Tronsmo A
Norwegian Crop Research Institute, Department of Plant Pathology, As, Norway.
Gene. 1996 May 24;171(1):123-7. doi: 10.1016/0378-1119(96)00031-5.
A full-length cDNA close, Th1433, (GenBank accession No. U24158), was isolated and characterized from the filamentous fungus, Trichoderma harzianum. The deduced amino acid (aa) sequence showed an acidic 30-kDa protein homologous to the 14-3-3 proteins, a family of putative kinase regulators originally characterized in mammalian brain tissue. The greatest homology, 71% identical aa, was found to BMH1, the corresponding protein from Saccharomyces cerevisiae and to the epsilon isoform from sheep brain. Southern analysis of genomic DNA indicated that Th1433 is a member of a small genomic family. At least two genes encoding 14-3-3-like proteins exist in T. harzianum. Northern analysis showed the highest level of expression during the first day after inoculation of the culture with conidial spores.
从丝状真菌哈茨木霉中分离并鉴定出一个全长 cDNA 克隆 Th1433（GenBank 登录号 U24158）。推导的氨基酸序列显示它是一个 30 kDa 的酸性蛋白，与 14-3-3 蛋白同源，14-3-3 蛋白是最初在哺乳动物脑组织中发现的一类假定的激酶调节剂家族。发现其与酿酒酵母的相应蛋白 BMH1 以及绵羊脑的 ε 亚型同源性最高，氨基酸同一性为 71%。基因组 DNA 的 Southern 分析表明 Th1433 是一个小基因组家族的成员。哈茨木霉中至少存在两个编码 14-3-3 样蛋白的基因。Northern 分析显示，在用分生孢子接种培养物后的第一天，表达水平最高。
