Kwak J, McCue L A, Kendrick K E
Department of Microbiology, Ohio State University, Columbus 43210, USA.
Gene. 1996 May 24;171(1):75-8. doi: 10.1016/0378-1119(96)00066-2.
The bldA gene (encoding tRNA(UUA)Leu) from Streptomyces griseus (Sg) was cloned by hybridization with bldA from Streptomyces coelicolor (Sc). Introduction of Sg bldA into Sc bldA mutants restored sporulation and actinorhodin production. Sporulation of a subset of Sg bald mutants, which produce no aerial mycelium or spores, was restored in the presence of bldA from Sc or Sg. The nucleotide sequences of the bldA alleles from two such bald mutants revealed point mutations in the anticodon stem and the T psi C stem.
通过与天蓝色链霉菌（Sc）的bldA基因杂交，克隆了灰色链霉菌（Sg）的bldA基因（编码tRNA(UUA)Leu）。将Sg的bldA基因导入Sc的bldA突变体中可恢复孢子形成和放线紫红素的产生。在存在Sc或Sg的bldA基因时，不产生气生菌丝或孢子的一部分Sg秃头突变体的孢子形成得以恢复。来自两个此类秃头突变体的bldA等位基因的核苷酸序列显示在反密码子茎和TψC茎中存在点突变。
