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致病性大肠杆菌感染期间肌球蛋白轻链在不同位点的磷酸化及其与细胞骨架的关联。

Phosphorylation of myosin light chain at distinct sites and its association with the cytoskeleton during enteropathogenic Escherichia coli infection.

作者信息

Manjarrez-Hernandez H A, Baldwin T J, Williams P H, Haigh R, Knutton S, Aitken A

机构信息

Laboratory of Protein Structure, National Institute for Medical Structure, London, United Kingdom.

出版信息

Infect Immun. 1996 Jun;64(6):2368-70. doi: 10.1128/iai.64.6.2368-2370.1996.

DOI:10.1128/iai.64.6.2368-2370.1996
PMID:8675355
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC174084/
Abstract

Myosin light chain, the most prominent host cell phosphoprotein during adhesion of enteropathogenic Escherichia coli to cultured HEp-2 cells, was shown to be distributed between cytosolic and cytoskeletal cell fractions; its association with the cytoskeletal fraction increased with time of enteropathogenic E. coli incubation. Phosphopeptide mapping indicated cytosolic and cytoskeletal myosin light chain phosphorylation at different sites by protein kinase C and myosin light chain kinase.

摘要

肌球蛋白轻链是肠道致病性大肠杆菌黏附培养的HEp-2细胞过程中最显著的宿主细胞磷蛋白，它分布于胞质和细胞骨架部分；随着肠道致病性大肠杆菌孵育时间的延长，它与细胞骨架部分的结合增加。磷酸肽图谱分析表明，胞质和细胞骨架中的肌球蛋白轻链在不同位点被蛋白激酶C和肌球蛋白轻链激酶磷酸化。

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本文引用的文献

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Enteropathogenic Escherichia coli adherence to intestinal epithelial monolayers diminishes barrier function.肠道致病性大肠杆菌对肠道上皮单层的黏附会削弱屏障功能。
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Phorbol ester-induced activation of human platelets is associated with protein kinase C phosphorylation of myosin light chains.佛波酯诱导的人血小板激活与肌球蛋白轻链的蛋白激酶C磷酸化有关。
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