致病性大肠杆菌感染期间肌球蛋白轻链在不同位点的磷酸化及其与细胞骨架的关联。
Phosphorylation of myosin light chain at distinct sites and its association with the cytoskeleton during enteropathogenic Escherichia coli infection.
作者信息
Manjarrez-Hernandez H A, Baldwin T J, Williams P H, Haigh R, Knutton S, Aitken A
机构信息
Laboratory of Protein Structure, National Institute for Medical Structure, London, United Kingdom.
出版信息
Infect Immun. 1996 Jun;64(6):2368-70. doi: 10.1128/iai.64.6.2368-2370.1996.
Abstract
Myosin light chain, the most prominent host cell phosphoprotein during adhesion of enteropathogenic Escherichia coli to cultured HEp-2 cells, was shown to be distributed between cytosolic and cytoskeletal cell fractions; its association with the cytoskeletal fraction increased with time of enteropathogenic E. coli incubation. Phosphopeptide mapping indicated cytosolic and cytoskeletal myosin light chain phosphorylation at different sites by protein kinase C and myosin light chain kinase.
摘要
肌球蛋白轻链是肠道致病性大肠杆菌黏附培养的HEp-2细胞过程中最显著的宿主细胞磷蛋白，它分布于胞质和细胞骨架部分；随着肠道致病性大肠杆菌孵育时间的延长，它与细胞骨架部分的结合增加。磷酸肽图谱分析表明，胞质和细胞骨架中的肌球蛋白轻链在不同位点被蛋白激酶C和肌球蛋白轻链激酶磷酸化。
相似文献
1
Phosphorylation of myosin light chain at distinct sites and its association with the cytoskeleton during enteropathogenic Escherichia coli infection.致病性大肠杆菌感染期间肌球蛋白轻链在不同位点的磷酸化及其与细胞骨架的关联。
Infect Immun. 1996 Jun;64(6):2368-70. doi: 10.1128/iai.64.6.2368-2370.1996.
2
Shear stress-induced myosin association with cytoskeleton and phosphorylation in human platelets.
Life Sci. 1997;60(11):PL181-91. doi: 10.1016/s0024-3205(97)00017-9.
3
Modulation of myosin light-chain phosphorylation by p21-activated kinase 1 in Escherichia coli invasion of human brain microvascular endothelial cells.p21激活激酶1对肌球蛋白轻链磷酸化的调节在大肠杆菌侵袭人脑血管内皮细胞中的作用
Infect Immun. 2003 May;71(5):2787-97. doi: 10.1128/IAI.71.5.2787-2797.2003.
4
Regulatory light chain phosphorylation and the assembly of myosin II into the cytoskeleton of microcapillary endothelial cells.调节性轻链磷酸化以及肌球蛋白II组装入微毛细血管内皮细胞的细胞骨架。
Cell Motil Cytoskeleton. 1999;43(3):255-68. doi: 10.1002/(SICI)1097-0169(1999)43:3<255::AID-CM8>3.0.CO;2-T.
5
Enteropathogenic Escherichia coli-induced myosin light chain phosphorylation alters intestinal epithelial permeability.肠致病性大肠杆菌诱导的肌球蛋白轻链磷酸化改变肠道上皮通透性。
Gastroenterology. 1997 Dec;113(6):1873-82. doi: 10.1016/s0016-5085(97)70006-4.
6
Purification of a 20 kDa phosphoprotein from epithelial cells and identification as a myosin light chain. Phosphorylation induced by enteropathogenic Escherichia coli and phorbol ester.
FEBS Lett. 1991 Nov 4;292(1-2):121-7. doi: 10.1016/0014-5793(91)80848-w.
7
Role of Rac1 in Escherichia coli K1 invasion of human brain microvascular endothelial cells.Rac1在大肠杆菌K1侵袭人脑血管内皮细胞中的作用
Microbes Infect. 2006 Feb;8(2):460-9. doi: 10.1016/j.micinf.2005.07.012. Epub 2005 Sep 16.
8
Increased phosphorylation of myosin light chain prevents in vitro decidualization.肌球蛋白轻链磷酸化增加会阻止体外蜕膜化。
Endocrinology. 2007 Jul;148(7):3176-84. doi: 10.1210/en.2006-1673. Epub 2007 Apr 5.
9
Increases in phosphorylation of the myosin II heavy chain, but not regulatory light chains, correlate with insulin secretion in rat pancreatic islets and RINm5F cells.在大鼠胰岛和RINm5F细胞中，肌球蛋白II重链而非调节轻链的磷酸化增加与胰岛素分泌相关。
Diabetes. 1999 Dec;48(12):2383-9. doi: 10.2337/diabetes.48.12.2383.
10
aPKCζ affects directed cell migration through the regulation of myosin light chain phosphorylation.aPKCζ 通过调节肌球蛋白轻链磷酸化影响定向细胞迁移。
Cell Adh Migr. 2017 Jul 4;11(4):347-359. doi: 10.1080/19336918.2016.1225631. Epub 2016 Aug 19.
引用本文的文献
1
Modulation of Intestinal Paracellular Transport by Bacterial Pathogens.细菌病原体对肠道细胞旁通透性的调节。
Compr Physiol. 2018 Mar 25;8(2):823-842. doi: 10.1002/cphy.c170034.
2
Post-infectious irritable bowel syndrome: mechanistic insights into chronic disturbances following enteric infection.感染后肠易激综合征：对肠道感染后慢性紊乱的机制性见解。
World J Gastroenterol. 2014 Apr 14;20(14):3976-85. doi: 10.3748/wjg.v20.i14.3976.
3
Chlamydia trachomatis inclusion membrane protein CT228 recruits elements of the myosin phosphatase pathway to regulate release mechanisms.沙眼衣原体包涵体膜蛋白 CT228 募集肌球蛋白磷酸酶途径的元件以调节释放机制。
Cell Rep. 2013 Jun 27;3(6):1921-31. doi: 10.1016/j.celrep.2013.04.027. Epub 2013 May 30.
4
Enteropathogenic E. coli effectors EspG1/G2 disrupt tight junctions: new roles and mechanisms.肠致病性大肠杆菌效应因子 EspG1/G2 破坏紧密连接：新的作用和机制。
Ann N Y Acad Sci. 2012 Jul;1258:149-58. doi: 10.1111/j.1749-6632.2012.06563.x.
5
Infectious diarrhea: Cellular and molecular mechanisms.感染性腹泻：细胞与分子机制
Gut Microbes. 2010 Jan;1(1):4-21. doi: 10.4161/gmic.1.1.11036.
6
Enteropathogenic and enterohemorrhagic Escherichia coli infections: translocation, translocation, translocation.肠道致病性和肠出血性大肠杆菌感染：易位，易位，易位。
Infect Immun. 2005 May;73(5):2573-85. doi: 10.1128/IAI.73.5.2573-2585.2005.
7
Modulation of myosin light-chain phosphorylation by p21-activated kinase 1 in Escherichia coli invasion of human brain microvascular endothelial cells.p21激活激酶1对肌球蛋白轻链磷酸化的调节在大肠杆菌侵袭人脑血管内皮细胞中的作用
Infect Immun. 2003 May;71(5):2787-97. doi: 10.1128/IAI.71.5.2787-2797.2003.
8
Enteropathogenic Escherichia coli activates ezrin, which participates in disruption of tight junction barrier function.肠道致病性大肠杆菌激活埃兹蛋白，该蛋白参与紧密连接屏障功能的破坏。
Infect Immun. 2001 Sep;69(9):5679-88. doi: 10.1128/IAI.69.9.5679-5688.2001.
9
MLCK-A, an unconventional myosin light chain kinase from Dictyostelium, is activated by a cGMP-dependent pathway.肌球蛋白轻链激酶-A（MLCK-A）是一种来自盘基网柄菌的非常规肌球蛋白轻链激酶，它通过一种环鸟苷酸（cGMP）依赖性途径被激活。
Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13000-5. doi: 10.1073/pnas.95.22.13000.
10
Increased levels of intracellular calcium are not required for the formation of attaching and effacing lesions by enteropathogenic and enterohemorrhagic Escherichia coli.肠道致病性大肠杆菌和肠出血性大肠杆菌形成紧密黏附损伤并不需要细胞内钙水平升高。
Infect Immun. 1998 Aug;66(8):3900-8. doi: 10.1128/IAI.66.8.3900-3908.1998.
本文引用的文献
1
Enteropathogenic Escherichia coli adherence to intestinal epithelial monolayers diminishes barrier function.肠道致病性大肠杆菌对肠道上皮单层的黏附会削弱屏障功能。
Am J Physiol. 1995 Feb;268(2 Pt 1):G374-9. doi: 10.1152/ajpgi.1995.268.2.G374.
2
Role of phosphorylation in mediating the association of myosin with the cytoskeletal structures of human platelets.磷酸化在介导肌球蛋白与人血小板细胞骨架结构结合中的作用。
J Biol Chem. 1982 Apr 25;257(8):4120-6.
3
Phorbol ester-induced activation of human platelets is associated with protein kinase C phosphorylation of myosin light chains.佛波酯诱导的人血小板激活与肌球蛋白轻链的蛋白激酶C磷酸化有关。
Nature. 1983;306(5942):490-2. doi: 10.1038/306490a0.
4
Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.劳氏肉瘤病毒的转化基因产物使酪氨酸磷酸化。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5. doi: 10.1073/pnas.77.3.1311.
5
Protein kinase C modulates in vitro phosphorylation of the smooth muscle heavy meromyosin by myosin light chain kinase.蛋白激酶C调节肌球蛋白轻链激酶对平滑肌重酶解肌球蛋白的体外磷酸化作用。
J Biol Chem. 1984 Jul 25;259(14):8808-14.
6
Myosin light chain phosphorylation in fibroblast shape change, detachment and patching.成纤维细胞形态改变、脱离及斑块形成过程中的肌球蛋白轻链磷酸化
Eur J Cell Biol. 1986 Oct;42(1):10-6.
7
Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa.肠致病性大肠杆菌对人肠道肠上皮细胞和培养的人肠黏膜的黏附作用。
Infect Immun. 1987 Jan;55(1):69-77. doi: 10.1128/iai.55.1.69-77.1987.
8
Evaluation of myosin light chain phosphorylation in isolated pancreatic acini.分离的胰腺腺泡中肌球蛋白轻链磷酸化的评估
Am J Physiol. 1988 Jan;254(1 Pt 1):G130-4. doi: 10.1152/ajpgi.1988.254.1.G130.
9
Regulation of actin microfilament integrity in living nonmuscle cells by the cAMP-dependent protein kinase and the myosin light chain kinase.环磷酸腺苷依赖性蛋白激酶和肌球蛋白轻链激酶对活的非肌肉细胞中肌动蛋白微丝完整性的调节
J Cell Biol. 1988 Jun;106(6):1955-71. doi: 10.1083/jcb.106.6.1955.
10
Tight junction dynamics: is paracellular transport regulated?
Cell. 1988 May 20;53(4):497-8. doi: 10.1016/0092-8674(88)90562-4.
Zotero 插件