Dolgiĭ N L, Degtiar' R G, Gulyĭ M F
Ukr Biokhim Zh. 1977 Mar-Apr;49(2):90-5.
The article deals with conditions for splitting the Penicillium vitale glucosooxidase molecule into apoenzyme and coenzyme, for reconstruction of the enzyme from apoenzyme and FAD as well as for the effect of some factors ("labilizing" fraction, dithiotreitol) on it. Specific activity of the reconstructed enzyme is on the average 85% of the initial enzyme specific activity: "the labilizing" fraction inhibits reconstruction of glucosooxidase from apoenzyme and FAD, that is due to oxidation of the apoenzyme sulphydryl groups. Possible participation of sulphydryl groups in adding FAD to apoenzyme is under discussion.
本文探讨了将产黄青霉葡萄糖氧化酶分子拆分为脱辅酶和辅酶的条件、由脱辅酶和黄素腺嘌呤二核苷酸（FAD）重构该酶的条件以及某些因素（“不稳定”组分、二硫苏糖醇）对其的影响。重构酶的比活性平均为初始酶比活性的85%：“不稳定”组分抑制了由脱辅酶和FAD重构葡萄糖氧化酶，这是由于脱辅酶巯基的氧化。文中讨论了巯基在将FAD添加到脱辅酶过程中可能的作用。
