Buchwalder A, Welch S K, Peroutka S J
Palo Alto Institute of Molecular Medicine, Mountain View, Calif, USA.
Headache. 1996 Apr;36(4):254-8. doi: 10.1046/j.1526-4610.1996.3604254.x.
Several lines of investigation suggest that the serotonergic system may be involved in the pathogenesis of migraine. In particular, drugs which block 5-HT2 receptor subtypes appear to be effective migraine prophylactic agents. Therefore, chromosomal DNA regions overlapping the 5-HT2A (13q14-q22) and 5-HT2C(Xq22-25) receptor loci were analyzed for possible linkage to the clinical diagnosis of migraine. No evidence for linkage to either chromosomal region was found, although a small subset of migrainous families showed positive likelihood of odds (LOD) scores. However, a homogeneity (HOMOG) analysis provided no statistical evidence for locus heterogeneity. The coding region of the 5-HT2A and 5-HT2C receptor genes was also analyzed in migraine patients and unaffected controls using polmerase chain reaction and direct sequencing. No mutations were found in the deduced amino acid sequence of either receptor in the sample of migraineurs tested. These results indicate that DNA-based mutations in the 5-HT2A and 5-HT2C receptors are not generally involved in the pathogenesis of migraine.
多项研究表明，血清素能系统可能参与偏头痛的发病机制。特别是，阻断5-HT2受体亚型的药物似乎是有效的偏头痛预防剂。因此，对与5-HT2A（13q14-q22）和5-HT2C（Xq22-25）受体基因座重叠的染色体DNA区域进行分析，以确定其与偏头痛临床诊断的可能连锁关系。尽管一小部分偏头痛家族显示出阳性优势对数（LOD）评分，但未发现与任何一个染色体区域连锁的证据。然而，同质性（HOMOG）分析未提供基因座异质性的统计证据。还使用聚合酶链反应和直接测序对偏头痛患者和未受影响的对照者的5-HT2A和5-HT2C受体基因的编码区进行了分析。在所测试的偏头痛患者样本中，两种受体的推导氨基酸序列均未发现突变。这些结果表明，5-HT2A和5-HT2C受体基于DNA的突变通常不参与偏头痛的发病机制。
