Pall A A, Howie A J, Adu D, Richards G M, Inward C D, Milford D V, Richards N T, Michael J, Taylor C M
Renal Research Laboratories, Queen Elizabeth Medical Centre, Edgbaston, Birmingham.
J Clin Pathol. 1996 Mar;49(3):238-42. doi: 10.1136/jcp.49.3.238.
To study the expression of cell adhesion molecules in the renal biopsy specimens of patients with systemic vasculitis and Henoch-Schönlein purpura (HSP); to correlate this with the severity of glomerular inflammation.
Renal biopsy specimens obtained from eight patients with untreated systemic vasculitis (four with Wegener's granulomatosis and four with microscopic polyarteritis), eight with HSP and nine controls (four with normal histopathology and five with thin glomerular basement membrane disease) were stained using the alkaline phosphatase anti-alkaline phosphatase method with monoclonal antibodies directed against intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and E-selectin.
Biopsy specimens of normal kidneys expressed ICAM-1 in glomerular endocapillary cells, Bowman's capsule epithelium, interstitial cells and interstitial vascular endothelium, and VCAM-1 in Bowman's capsule epithelium, proximal tubular epithelium and interstitial vascular endothelium. No staining with antibody directed against E-selectin was seen in any of the biopsy specimens. Biopsy specimens of patients with a vasculitic glomerulonephritis (segmental necrotising glomerulonephritis) expressed VCAM-1 in glomerular endocapillary cells (four of eight patients with systemic vasculitis; two of eight patients with HSP). In patients with a systemic vasculitis glomerular VCAM-1 expression was associated with a more severe renal lesoin (44, 50, 60, and 65% of glomeruli involved) than in those not showing glomerular VCAM-1 expression (3, 3, 11, and 39% of glomeruli involved).
Expression of VCAM-1 by glomerular endocapillary cells in renal biopsy specimens raises the possibility that recruitment of VLA-4 bearing leucocytes may contribute to glomerular injury in Wegener's granulomatosis and microscopic polyarteritis.
研究细胞黏附分子在系统性血管炎和过敏性紫癜（HSP）患者肾活检标本中的表达，并将其与肾小球炎症的严重程度相关联。
使用抗碱性磷酸酶法，用针对细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1（VCAM-1）和E-选择素的单克隆抗体，对8例未经治疗的系统性血管炎患者（4例韦格纳肉芽肿和4例显微镜下多动脉炎）、8例HSP患者以及9例对照者（4例组织病理学正常和5例薄肾小球基底膜病）的肾活检标本进行染色。
正常肾脏活检标本中，ICAM-1在肾小球毛细血管内皮细胞、鲍曼囊上皮细胞、间质细胞和间质血管内皮细胞中表达，VCAM-1在鲍曼囊上皮细胞、近端肾小管上皮细胞和间质血管内皮细胞中表达。所有活检标本均未见到针对E-选择素的抗体染色。血管炎性肾小球肾炎（节段性坏死性肾小球肾炎）患者的活检标本中，肾小球毛细血管内皮细胞表达VCAM-1（8例系统性血管炎患者中的4例；8例HSP患者中的2例）。在系统性血管炎患者中，肾小球VCAM-1表达与更严重的肾脏病变相关（肾小球受累比例分别为44%、50%、60%和65%），而未显示肾小球VCAM-1表达的患者肾小球受累比例为3%、3%、11%和39%。
肾活检标本中肾小球毛细血管内皮细胞表达VCAM-1提示，携带VLA-4的白细胞募集可能在韦格纳肉芽肿和显微镜下多动脉炎的肾小球损伤中起作用。
