Urano Y, Sasaki S, Ninomiya Y, Oura H, Arase S
Department of Dermatology, School of Medicine, University of Tokushima, Japan.
J Dermatol. 1996 May;23(5):365-8. doi: 10.1111/j.1346-8138.1996.tb04033.x.
We examined 9 Japanese cases of porokeratosis (4 of the plaque type, 2 of disseminated superficial actinic porokeratosis, 2 of disseminated superficial porokeratosis, and one of giant porokeratosis) for the expression of p53 tumor suppressor protein immunohistochemically, using two anti-p53 antibodies, CM1 and DO1. The same results were obtained with both antibodies. The epidermis central to the cornoid lamellae was positive in 8 of 9 specimens. On the other hand, the peripheral epidermis was positive in 2 of the 9 cases. The epidermis beneath the cornoid lamellae was positive in 3 of the 9 cases. The frequency of p53 positivity was significantly higher in the epidermis central to cornoid lamellae over that beneath or peripheral to them (Fisher's exact probability test, p < 0.05). The majority of squamous cell carcinoma cells arising on giant porokeratosis stained with CM1 and DO1. These data may suggest that the abnormal p53 expression has some relevance to the skin carcinogenesis of porokeratosis.
我们使用两种抗p53抗体CM1和DO1，通过免疫组织化学方法检测了9例日本汗孔角化症患者（4例斑块型、2例播散性浅表性光化性汗孔角化症、2例播散性浅表性汗孔角化症和1例巨大型汗孔角化症）中p53肿瘤抑制蛋白的表达情况。两种抗体得出了相同的结果。9个标本中有8个标本的鸡眼样板层中心的表皮呈阳性。另一方面，9例中有2例周边表皮呈阳性。9例中有3例鸡眼样板层下方的表皮呈阳性。鸡眼样板层中心的表皮中p53阳性频率显著高于其下方或周边的表皮（Fisher精确概率检验，p<0.05）。巨大型汗孔角化症发生的大多数鳞状细胞癌细胞被CM1和DO1染色。这些数据可能表明p53异常表达与汗孔角化症的皮肤癌变有一定关联。
