Rustenbeck I, Lenzen S
Institute of Pharmacology and Toxicology, University of Göttingen, Germany.
J Biochem Biophys Methods. 1996 Feb 5;31(3-4):105-12. doi: 10.1016/0165-022x(95)00020-r.
A device is described to perform potentiometric measurements with ion-sensitive electrodes in stirred photometric cuvettes. Its design allows to make additions to the reaction medium with microliter syringes during measurements. Originally, this plug-in electrode was designed to register the free Ca2+ concentrations in the incubation medium of mitochondrial suspensions during measurements of the free matrix Ca2+ concentration with a permeant fluorescent Ca2+ indicator. However, numerous other applications can be easily realized, such as the combination of mitochondrial light-scattering measurements and ion-transport measurements, the combination of the permeabilized cell technique with fluorescence measurements of intracellular organelles or simply the calibration of the fluorescence of Ca2+ indicators with a Ca2+ ion-sensitive minielectrode. Compared with the use of a second fluorescent indicator the use of an electrode has the advantage that the signal can be transformed into ion concentrations already during the measurements.
描述了一种用于在搅拌的光度比色皿中使用离子敏感电极进行电位测量的装置。其设计允许在测量期间用微升注射器向反应介质中添加物质。最初，这种插入式电极旨在在用渗透性荧光钙指示剂测量游离基质钙浓度期间记录线粒体悬浮液孵育介质中的游离钙离子浓度。然而，许多其他应用也可以轻松实现，例如线粒体光散射测量与离子转运测量的结合、通透细胞技术与细胞内细胞器荧光测量的结合，或者简单地用钙离子敏感微电极校准钙指示剂的荧光。与使用第二种荧光指示剂相比，使用电极的优点是在测量过程中信号就可以转化为离子浓度。
