Overman S A, Tsuboi M, Thomas G J
Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, 64110, USA.
J Mol Biol. 1996 Jun 14;259(3):331-6. doi: 10.1006/jmbi.1996.0323.
The Ff filamentous viruses (fd, M13, f1) are important models for membrane protein assembly and are used extensively as cloning vectors and vehicles for peptide display. The thread-like virion (approximately 6 nm x 880 nm) comprises a single-stranded DNA genome sheathed by approximately 2700 copies of a 50-residue alpha-helical subunit (pVIII). The average inclination of pVIII subunits from the virion axis has been determined experimentally by polarized Raman microspectroscopy of oriented fd fibers, using the amide I Raman tensors obtained from a peptide single crystal. It is found that the average tilt angle, theta, between the pVIII alpha-helix and the virion axis falls within the range 13< theta <20 degrees.
Ff丝状病毒（fd、M13、f1）是膜蛋白组装的重要模型，被广泛用作克隆载体和肽展示载体。丝状病毒粒子（约6纳米×880纳米）由单链DNA基因组组成，该基因组被约2700个50个残基的α螺旋亚基（pVIII）包裹。通过对取向fd纤维进行偏振拉曼光谱实验，并利用从肽单晶获得的酰胺I拉曼张量，已通过实验确定了pVIII亚基相对于病毒粒子轴的平均倾斜度。发现pVIIIα螺旋与病毒粒子轴之间的平均倾斜角θ在13＜θ＜20度范围内。
