Taylor G A
Russell H. Morgan Department of Radiology, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287, USA.
Pediatr Radiol. 1996;26(4):282-6. doi: 10.1007/BF01372114.
To establish the feasibility of determining changes in regional cerebral blood flow using amplitude-mode color Doppler ultrasound in the newborn lamb.
Regional cerebral blood flow was modified by intrastriatal injection of N -methyl-D-aspartic acid (NMDA) in two newborn lambs and compared with that in a control animal receiving only intrastriatal saline. Serial color Doppler ultrasound energy images were obtained in coronal projection at the level of the foramen of Monro, before and at 20, 40, and 60 min after NMDA or saline injection using a 7.0-MHz linear transducer. Real-time images were obtained and transferred onto a computer workstation. Regions of interest were drawn over each striatum and cerebral hemisphere and were analyzed for mean pixel intensity (MPI) at each interval. Paired regional cerebral blood flow (r-CBF) determinations (radiolabeled microsphere technique) were also obtained in every animal. MPI and r-CBF for each anatomic region were compared using linear regression.
Mean color pixel intensities increased significantly in the ipsilateral basal ganglia and cerebral hemisphere (p < 0.0001) in both animals injected with NMDA, but not in the animal injected with normal saline (p < 0.63). A strong and significant linear correlation was found between MPI and r-CBF for striatum (r = 0.89, p < 0.0001) and cerebral hemisphere (r = 0.85, p < 0.0001).
Amplitude-mode color Doppler ultrasound shows potential for technically simple determination of serial changes in regional cerebral blood flow in infants.
探讨在新生羔羊中使用振幅模式彩色多普勒超声测定局部脑血流变化的可行性。
对两只新生羔羊纹状体内注射N-甲基-D-天冬氨酸（NMDA）以改变局部脑血流，并与仅接受纹状体内注射生理盐水的对照动物进行比较。在注射NMDA或生理盐水之前以及之后20、40和60分钟，使用7.0兆赫线性换能器在Monro孔水平获取冠状面的系列彩色多普勒超声能量图像。获取实时图像并传输到计算机工作站。在每个纹状体和脑半球上绘制感兴趣区域，并分析每个时间间隔的平均像素强度（MPI）。每只动物还采用放射性微球技术进行配对局部脑血流（r-CBF）测定。使用线性回归比较每个解剖区域的MPI和r-CBF。
在两只注射NMDA的动物中，同侧基底神经节和脑半球的平均彩色像素强度显著增加（p<0.0001），而在注射生理盐水的动物中则未增加（p<0.63）。纹状体（r = 0.89，p<0.0001）和脑半球（r = 0.85，p<0.0001）的MPI与r-CBF之间存在强且显著的线性相关性。
振幅模式彩色多普勒超声在技术上有可能简单地测定婴儿局部脑血流的系列变化。
