Jaffe R L
Environmental Toxicology Laboratory, Long Island City, New York 11101, USA.
Toxicol Ind Health. 1995 Sep-Oct;11(5):543-58. doi: 10.1177/074823379501100507.
A simple test for measuring cytotoxic agents has been developed using the flagellate phenotype of Tetramitus rostratus. The test measures dose-dependent inhibition of cell division by individual agents such as 4-nitroquinoline-N-oxide and other mutagens. Dose-response data are given also for mixtures including coal tar pitch condensate, centrifuged particles obtained from tap water, and water concentrates prepared with XAD-2 resin. Because Tetramitus flagellates have a gullet and are particle-feeders, the assay allows for cytotoxic measurements of whole particles without prior extraction or solvent substitution procedures. The cytotoxic activities observed may reflect genotoxic activity, since all chemicals that produced a positive response are genotoxic in other test systems.
利用罗氏四膜虫的鞭毛虫表型开发了一种用于检测细胞毒性剂的简单测试方法。该测试可测量诸如4-硝基喹啉-N-氧化物等单个试剂以及其他诱变剂对细胞分裂的剂量依赖性抑制作用。还给出了包括煤焦油沥青浓缩物、自来水中获得的离心颗粒以及用XAD-2树脂制备的水浓缩物等混合物的剂量反应数据。由于四膜虫鞭毛虫有一个食管并且是颗粒摄食者，该检测方法无需事先提取或溶剂置换程序就能对整个颗粒进行细胞毒性测量。观察到的细胞毒性活性可能反映了遗传毒性活性，因为所有产生阳性反应的化学物质在其他测试系统中都是遗传毒性的。
