Ghyka G, Stoian N, Sorodoc C, Peiulescu P, Osman J, Puca D
Virologie. 1977 Apr-Jun;28(2):111-5.
The authors establish the optimal conditions (protein concentration, temperature) for the coupling of purified mycoplasma antigen to sheep red blood cells, in order to obtain an antigenic complex stable for at least 1 year. This comples allows specific determination by passive hemagglutination--a simple and rapid technique--of anti-mycoplasma antibodies in the sera of men or animals suspected of mycoplasma infections. Under the stated experimental conditions, antibody titers equal to or higher than 1/128 indicate an infection with M. pneumoniae.
作者确定了纯化的支原体抗原与绵羊红细胞偶联的最佳条件（蛋白质浓度、温度），以获得至少稳定1年的抗原复合物。这种复合物能够通过被动血凝反应（一种简单快速的技术）对疑似支原体感染的人或动物血清中的抗支原体抗体进行特异性检测。在规定的实验条件下，抗体效价等于或高于1/128表明感染了肺炎支原体。
