Ohki S, Onda M, Nomizu T, Tsuchiya A, Ami H, Fukushima T, Takita K, Kanazawa M, Watanabe F, Andoh Y, Yoshida T, Abe R
Second Dept. of Surgery, Fukushima Medical College, Japan.
Gan To Kagaku Ryoho. 1996 Jun;23 Suppl 2:154-8.
A 18-year-old woman underwent total colectomy for familial adenomatous polyposis. In order to clarify the significance of K-ras mutations in early colorectal carcinogenesis, K-ras mutations were analyzed in multiple adenomas by PCR-SSCP method. A total of 256 adenomas were found throughout the entire colon and rectum, and the distribution was a sparse type. The correlation between K-ras gene and clinicopathological factors was examined in 90 adenomas. There was no correlation among K-ras mutations and anatomical distribution, or morphological classification, but K-ras mutation was more frequent in severe compared with slight atypia. We investigated the correlation between the size of adenoma in the horizontal and vertical directions and K-ras mutation. K-ras mutation was more frequent in the horizontal size greater than 6 mm in diameter, and also more frequent in vertical size greater than 20 mm in height. It was concluded that the adenomas detecting K-ras mutations might have proliferating potential, and would be applied to determine polypectomy.
一名18岁女性因家族性腺瘤性息肉病接受了全结肠切除术。为了阐明K-ras突变在早期结直肠癌发生中的意义，采用聚合酶链反应-单链构象多态性（PCR-SSCP）方法对多个腺瘤中的K-ras突变进行了分析。在整个结肠和直肠共发现256个腺瘤，其分布为稀疏型。在90个腺瘤中检测了K-ras基因与临床病理因素之间的相关性。K-ras突变与解剖分布或形态学分类之间无相关性，但与轻度异型增生相比，重度异型增生中K-ras突变更为常见。我们研究了腺瘤在水平和垂直方向的大小与K-ras突变之间的相关性。直径大于6 mm的水平大小腺瘤以及高度大于20 mm的垂直大小腺瘤中K-ras突变更为常见。得出的结论是，检测到K-ras突变的腺瘤可能具有增殖潜能，可用于确定息肉切除术。
