牛磺酸和钙对生物膜直接作用的证据。一项31P核磁共振与电子自旋共振的联合研究。

Evidence of a direct action of taurine and calcium on biological membranes. A combined study of 31P-nuclear magnetic resonance and electron spin resonance.

作者信息

Nakashima T, Shima T, Sakai M, Yama H, Mitsuyoshi H, Inaba K, Matsumoto N, Sakamoto Y, Kashima K, Nishikawa H

机构信息

Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.

出版信息

Biochem Pharmacol. 1996 Jul 12;52(1):173-6. doi: 10.1016/0006-2952(96)00166-9.

DOI:10.1016/0006-2952(96)00166-9

PMID:8678903

Abstract

To determine the actions of taurine and calcium on biological membranes, the effects of these compounds on the mobility of phospholipids of resealed and sonicated ghosts of human erythrocytes were investigated, using 31P-NMR spectroscopy. In addition, the effects of taurine and calcium on lipid fluidity were investigated by ESR spectroscopy, using a spin-labeling method with 5-doxyl stearic acid. The mobility of the membranes decreased following treatment with 10 mM taurine, but coadministration of 2.5 mM calcium blocked this effect. The fluidity of the membranes was not changed following treatment with 10 mM taurine, but decreased following coadministration of 2.5 mM calcium. These actions of taurine and calcium on the dynamics of biological membranes might explain, in part, the observation that most of the pharmacological effects of taurine on mammalian organs occur in the presence of calcium ions.

摘要

为了确定牛磺酸和钙对生物膜的作用，利用31P-核磁共振波谱法研究了这些化合物对人红细胞重封和超声处理后的细胞膜磷脂流动性的影响。此外，采用5-硬脂酰基-二氧磷杂环己烷自旋标记法，通过电子顺磁共振波谱研究了牛磺酸和钙对脂质流动性的影响。用10 mM牛磺酸处理后，膜的流动性降低，但同时给予2.5 mM钙可阻断这种作用。用10 mM牛磺酸处理后，膜的流动性没有改变，但同时给予2.5 mM钙后，膜的流动性降低。牛磺酸和钙对生物膜动力学的这些作用可能部分解释了以下观察结果：牛磺酸对哺乳动物器官的大多数药理作用是在钙离子存在的情况下发生的。