使用嗜血杆菌属时，BBL Crystal ID试剂盒得出的异常但有用的结果

Anomalous but helpful findings from the BBL Crystal ID kit with Haemophilus spp.

作者信息

Hamilton-Miller J M, Shah S

机构信息

Department of Medical Microbiology, Royal Free Hospital School of Medicine, London, UK.

出版信息

Lett Appl Microbiol. 1996 Jul;23(1):47-8. doi: 10.1111/j.1472-765x.1996.tb00026.x.

DOI:10.1111/j.1472-765x.1996.tb00026.x

PMID:8679142

Abstract

Fourteen strains of Haemophilus (12 H. influenzae, 1 H. parainfluenzae and 1 H. aphrophilus) were processed in BBL Crystal ID Enteric/Nonfermenter, API 20E and API 20NE kits, to determine whether the BBL kit misidentifies, as API kits may do, Haemophilus spp. as Pasteurella spp. The 13 H. influenzae and H. parainfluenzae strains produced uninterpretable colour reactions in the Crystal kit, thus signalling that an inappropriate species had been tested. On the other hand, the API kits (especially 20NE) often confidently "identified' Haemophilus spp. as Pasteurella spp., giving no warning that this was a misidentification.

摘要

对14株嗜血杆菌（12株流感嗜血杆菌、1株副流感嗜血杆菌和1株嗜沫嗜血杆菌）使用BBL Crystal ID肠道菌/非发酵菌鉴定试剂盒、API 20E鉴定试剂盒和API 20NE鉴定试剂盒进行检测，以确定BBL试剂盒是否会像API试剂盒那样将嗜血杆菌属误鉴定为巴斯德菌属。13株流感嗜血杆菌和副流感嗜血杆菌菌株在Crystal试剂盒中产生了无法解读的颜色反应，这表明检测的是不适当的菌种。另一方面，API试剂盒（尤其是20NE）常常自信地将嗜血杆菌属“鉴定”为巴斯德菌属，而没有给出这是错误鉴定的警示。