Olafsen T, Bruland O S, Zalutsky M R, Sandlie I
Division of Molecular and Cell Biology, University of Oslo, Oslo, Norway.
Acta Oncol. 1996;35(3):297-301. doi: 10.3109/02841869609101644.
The variable (V) genes of TP-1 and TP-3 MAbs have been cloned and sequenced. Because of the potential use of these antibodies in the diagnosis and treatment of osteosarcoma, it is important to determine the presence and position of amino acid residues that may react with radiolabeling within the V domains. In this article, location of the tyrosine residues is determined using the knowledge of immunoglobulin structures in general. The TP-1V domains have a total of 19 tyrosines, whereas TP-3V domains have 18, with approximately half of these located within complementarity determining regions (CDRs). Thus, if equal reactivity of all tyrosines is assumed, smaller fragments of MAbs have a high probability of being radiolabeled at one of these sites with possible resultant loss of antigen binding.
TP-1和TP-3单克隆抗体的可变（V）基因已被克隆和测序。由于这些抗体在骨肉瘤诊断和治疗中的潜在用途，确定V结构域内可能与放射性标记反应的氨基酸残基的存在和位置非常重要。在本文中，利用一般免疫球蛋白结构的知识确定酪氨酸残基的位置。TP-1 V结构域共有19个酪氨酸，而TP-3 V结构域有18个，其中约一半位于互补决定区（CDR）内。因此，如果假设所有酪氨酸具有相同的反应性，单克隆抗体的较小片段很有可能在这些位点之一被放射性标记，从而可能导致抗原结合丧失。
