Slade A, Jones S, Jenkins A, Bootman J, Heath A, Kitchin P, Almond N
AIDS Collaborating Centre, National Institute of Biological Standards and Control, Herts, UK.
AIDS Res Hum Retroviruses. 1995 Dec;11(12):1509-11. doi: 10.1089/aid.1995.11.1509.
Two cynomolgus macaques were infected with a genetically complex challenge stock of simian immunodeficiency virus (SIVmac251-32H). One animal developed SIV-induced disease and was sacrificed at 16 months postinfection. The second remained healthy until it too was sacrificed at 20 months postinfection. The polymerase chain reaction (PCR) was used to amplify env gp120-coding sequences from provirus present in samples of blood, spleen, and inguinal lymph node taken from both animals on the day of sacrifice. The proviral burden present in each of the tissue samples was also determined using a quantitative PCR assay. The proviral burdens in the blood, spleen, and inguinal lymph node of the healthy animal (I225) were similar. This was not the case for animal I227, in which the burden in the inguinal lymph node was much higher than for blood or spleen. Phenogram analysis of the hypervariable V1 region of env revealed that the diversity of nucleotide sequences recovered from each tissue of both macaques were similar and overlapping. Some selected amino acid differences were observed that were specific for a tissue or one of the macaques. However, the results do not suggest that the overall evolution of env in provirus populations recovered from lymphoid tissues is distinct from that recovered from the blood.
两只食蟹猴感染了一种基因复杂的猿猴免疫缺陷病毒（SIVmac251 - 32H）攻击毒株。其中一只动物出现了SIV诱导的疾病，并在感染后16个月被处死。第二只动物一直保持健康，直到在感染后20个月也被处死。在处死当天，使用聚合酶链反应（PCR）从取自两只动物的血液、脾脏和腹股沟淋巴结样本中的前病毒中扩增env gp120编码序列。还使用定量PCR测定法确定每个组织样本中存在的前病毒载量。健康动物（I225）的血液、脾脏和腹股沟淋巴结中的前病毒载量相似。动物I227的情况并非如此，其腹股沟淋巴结中的载量远高于血液或脾脏中的载量。对env高变V1区的系统发育分析表明，从两只猕猴的每个组织中回收的核苷酸序列的多样性相似且重叠。观察到一些特定于某个组织或其中一只猕猴的选定氨基酸差异。然而，结果并不表明从淋巴组织中回收的前病毒群体中env的总体进化与从血液中回收的不同。
