Becker W, Kentrup H, Heukelbach J, Joost H G
Institut für Pharmakologie und Toxikologie, Medizinische Fakultät, RWTH Aachen, Germany.
Biochim Biophys Acta. 1996 Jun 5;1312(1):63-7. doi: 10.1016/0167-4889(96)00036-5.
A cDNA clone of a protein kinase with high similarity to the Clk (Cdc2-like kinases) subfamily was isolated from a rat brain library and characterized. Its deduced amino acid sequence exhibited a 99% identity with human Clk3 and was therefore designated rat Clk3. In addition to the protein kinase domain, the sequence (490 amino acids) comprises an N-terminal domain with a strikingly high portion of basic amino acids. A glutathione S-transferase fusion protein of Clk3 catalyzed autophosphorylation of the kinase but not phosphorylation of the exogenous substrates histone or casein. By Northern blot analysis of different rat tissues, mRNA of Clk3 was detected predominately in testis, suggesting that this kinase regulates a predominately testicular function.
从大鼠脑文库中分离并鉴定了一种与Clk（Cdc2样激酶）亚家族高度相似的蛋白激酶的cDNA克隆。其推导的氨基酸序列与人类Clk3具有99%的同一性，因此被命名为大鼠Clk3。除蛋白激酶结构域外，该序列（490个氨基酸）还包含一个N端结构域，其中碱性氨基酸的比例极高。Clk3的谷胱甘肽S-转移酶融合蛋白催化该激酶的自磷酸化，但不催化外源底物组蛋白或酪蛋白的磷酸化。通过对不同大鼠组织的Northern印迹分析，发现Clk3的mRNA主要在睾丸中检测到，这表明该激酶主要调节睾丸功能。
