[膜氧化还原链功能过程中形成的氧自由基在核DNA损伤中的作用]
[The role of oxygen radicals formed during function of the membrane redox chain, in damage of nuclear DNA].
作者信息
Peskin A V
机构信息
N.K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow.
出版信息
Biokhimiia. 1996 Jan;61(1):65-72.
Nuclear DNA damage resulting from incubation of isolated rat liver nuclei in the presence of NADPH or NADH and ethylenediamine tetraacetic acid, or diethylenetriamine pentaacetic acid, or desferoxamine complexes with Fe3+, has been studied. The degree of DNA damage was assessed by agarose electrophoresis. The observed damage was prevented by catalase but not superoxide dismutase. The mechanism of oxidative damage of nuclear DNA during membrane redox chains functioning is discussed.
研究了在存在烟酰胺腺嘌呤二核苷酸磷酸（NADPH）或烟酰胺腺嘌呤二核苷酸（NADH）以及乙二胺四乙酸（EDTA）、二乙烯三胺五乙酸（DTPA）或去铁胺与Fe3+形成的络合物的情况下，分离的大鼠肝细胞核温育所导致的核DNA损伤。通过琼脂糖电泳评估DNA损伤程度。过氧化氢酶可防止观察到的损伤，而超氧化物歧化酶则不能。文中讨论了核DNA在膜氧化还原链功能发挥过程中的氧化损伤机制。
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