Chi Z, Liu J, Xu P
Department of Microbiology, Shandong University, Jinan, China.
Chin J Biotechnol. 1995;11(3):171-6.
An improved process for high-concentration ethanol production from cooked corn starch by Saccharomyces sp. W4 has been well developed. Simultaneous cooking and liquefaction of corn starch were performed by using thermophilic alpha-amylase at 95 degrees to 105 degrees C. The mash was then saccharified at 60 degrees C by using high-efficiency glucoamylase. Saccharomyces sp. W4 could produce 18.3% (v/v) ethanol at 30 degrees C within 60 hrs, with 1.2% reducing sugar and 4.1% total sugar remaining in the fermented mash. It was found that ammonium sulfate could accelerate the fermentation rate. When 0.9 g of ammonium sulfate was added to 280 mL of the fermentation media, 18.9% (v/v) ethanol could be reached in the mash after 50 hrs of fermentation, leaving 0.27% reducing sugar and 3.1% total sugar in the fermented media.
已成功开发出一种经改进的由酿酒酵母W4菌株从熟玉米淀粉生产高浓度乙醇的工艺。使用嗜热α-淀粉酶在95至105摄氏度下对玉米淀粉进行同步蒸煮和液化。然后使用高效糖化酶在60摄氏度下对醪液进行糖化。酿酒酵母W4菌株在30摄氏度下60小时内可产生18.3%（v/v）的乙醇，发酵醪液中残留1.2%的还原糖和4.1%的总糖。发现硫酸铵可加快发酵速率。当向280毫升发酵培养基中添加0.9克硫酸铵时，发酵50小时后醪液中乙醇含量可达18.9%（v/v），发酵培养基中残留0.27%的还原糖和3.1%的总糖。
