Na S, Shen T, Xiao W, Jia P
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Chin J Biotechnol. 1995;11(3):193-8.
High-voltage electroporation was performed to transfer plasmid DNA of pXZ10145 into different kinds of corynebacteria strains. A number of factors that affected transformation efficiency were investigated. Cells grown in the presence of 4% glycine and harvested in the early exponential growth phase (OD600 was about 0.2) were much more easily transformed. Transformation efficiency up to 8 x 10(6) transformants per microgram of plasmid DNA with homologously derived DNA was obtained. If heterologously derived DNA was used, transformation efficiency was 10(2) to 10(3) times lower than the former one.
采用高压电穿孔法将pXZ10145质粒DNA导入不同种类的棒状杆菌菌株中。研究了多种影响转化效率的因素。在4%甘氨酸存在下生长并在指数生长早期（OD600约为0.2）收获的细胞更易于转化。使用同源DNA时，每微克质粒DNA的转化效率可达8×10⁶个转化子。若使用异源DNA，转化效率比前者低10²至10³倍。
