Pribush A, Meyerstein D, Meyerstein N
Physiology Department, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Biorheology. 1996 Mar-Apr;33(2):139-51. doi: 10.1016/0006-355X(96)00012-1.
A method based on dielectric properties of cellular suspensions was developed to study red blood cell (RBC) aggregability. The time-dependent current in a Couette-type viscometer was recorded after abrupt stoppage of shearing. Since the current reaches steady state 2 min after the end of shearing, the observed effects were quantified by the relative current difference, delta Irel = (I(2min)-I5s)/I2min, where subscripts designate the time of measurements. delta Irel increases with hematocrit, plasma and fibrinogen concentration. The dependence of delta Irel and of RBC aggregability on the concentration of dextran were similar. The experimental data and their analysis indicate that in suspensions with aggregating media, the delta Irel value measured in the field of the beta-dispersion reflects the difference between the size of aggregates under steady-state conditions and that of dispersed particles 5 s after the end of shearing. Therefore, this value may serve as a measure of RBC aggregability.
开发了一种基于细胞悬浮液介电特性的方法来研究红细胞（RBC）的聚集性。在剪切突然停止后，记录了库埃特型粘度计中随时间变化的电流。由于电流在剪切结束后2分钟达到稳态，因此通过相对电流差ΔIrel = (I(2min)-I5s)/I2min对观察到的效应进行量化，其中下标表示测量时间。ΔIrel随血细胞比容、血浆和纤维蛋白原浓度的增加而增加。ΔIrel与RBC聚集性对右旋糖酐浓度的依赖性相似。实验数据及其分析表明，在含有聚集介质的悬浮液中，在β-分散场中测量的ΔIrel值反映了稳态条件下聚集体大小与剪切结束后5秒分散颗粒大小之间的差异。因此，该值可作为RBC聚集性的一种度量。
