体液中吲哚丙烯酰甘氨酸及其可能前体的高效液相色谱分析

High-performance liquid chromatographic profiling of indolylacryloylglycine and its possible precursors in body fluids.

作者信息

Marklová E, Fojtásková A

机构信息

Department of Paediatrics, Charles University Medical Faculty Hospital, Hradec Králové, Czech Republic.

出版信息

J Chromatogr A. 1996 Apr 12;730(1-2):133-7. doi: 10.1016/0021-9673(95)00943-4.

DOI:10.1016/0021-9673(95)00943-4

PMID:8680585

Abstract

Indolylacryloylglycine (IAcrGly) is one of the physiological components of urine, although its source and its role in the human organism have not yet been unambiguously established. Changes in the IAcrGly excretion level have been found under some physiological (age dependence, seasonal variations) and pathological (photodermatoses, muscle dystrophy, liver cirrhosis) conditions. The proposed method for IAcrGly, indolylacrylic acid and its possible precursors, namely indolyllactic and indolylpropionic acids, involves deproteinization and extraction of urine on a Sep Pak C18 cartridge. HPLC analysis was carried out using a DataApex liquid chromatograph, equipped with an LCD 2082 UV detector, signals being acquired with a CSW workstation. The chromatographic column was Spherisorb ODS, 5 microns (125 x 4 mm I.D.), the mobile phase for isocratic elution was ethanol-1% acetic acid (27:73) and the flow-rate was 0.7 ml/min. The lower response limit is about 1 mumol/l for all metabolites at 280 nm.

摘要

吲哚丙烯酰甘氨酸（IAcrGly）是尿液的生理成分之一，尽管其来源及其在人体中的作用尚未明确确定。在一些生理（年龄依赖性、季节变化）和病理（光皮肤病、肌肉营养不良、肝硬化）条件下，已发现IAcrGly排泄水平会发生变化。所提出的用于检测IAcrGly、吲哚丙烯酸及其可能的前体，即吲哚乳酸和吲哚丙酸的方法，包括在Sep Pak C18柱上对尿液进行脱蛋白和萃取。使用配备LCD 2082紫外检测器的DataApex液相色谱仪进行高效液相色谱分析，信号通过CSW工作站采集。色谱柱为5微米的Spherisorb ODS（内径125×4毫米），等度洗脱的流动相为乙醇 - 1%乙酸（27:73），流速为0.7毫升/分钟。在280纳米处，所有代谢物的最低响应限约为1微摩尔/升。