使用联用柱高效液相色谱系统分析源自血红蛋白加合物的苯并[a]蒽和苯并[a]芘的四氢四醇的初步实验。
Preliminary experiments for the analysis of tetrahydrotetrols of benz[a]anthracene and benzo[a]pyrene derived from their hemoglobin adducts using a coupled-column high-performance liquid chromatographic system.
作者信息
Hackl C, Lintelmann J, Sauerbrey R, Kettrup A
机构信息
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Okologische Chemie, Oberschleissheim, Germany.
出版信息
J Chromatogr A. 1996 Apr 12;730(1-2):153-9. doi: 10.1016/0021-9673(95)01183-8.
Improved technologies for the detection of polycyclic aromatic hydrocarbon adducts are required for human biomonitoring. Therefore, a coupled-column high-performance liquid chromatographic method, with system-integrated sample processing, has been developed and its applicability for determination of tetrahydrotetrols of polycyclic aromatic hydrocarbons in acid hydrolysates of human hemoglobin has been investigated. A novel column-switching technique applying "thermotransfer' is used to separate tetrahydrotetrols of benzo[a]pyrene and benz[a]anthracene more efficiently. Derivatives of polycyclic aromatic hydrocarbons from blood hydrolysates are concentrated on a pre-column and then transferred to the analytical column by applying an electrical current to heat the solvent eluting the pre-column. This method allows for rapid and quantitative transfer of the analytes from the pre-column to the analytical column, after HPLC-integrated sample processing.
人体生物监测需要改进多环芳烃加合物的检测技术。因此，已开发出一种具有系统集成样品处理功能的联用柱高效液相色谱法，并研究了其在测定人血红蛋白酸水解产物中多环芳烃四氢四醇含量方面的适用性。一种应用“热转移”的新型柱切换技术被用于更有效地分离苯并[a]芘和苯并[a]蒽的四氢四醇。血液水解产物中的多环芳烃衍生物先在预柱上富集，然后通过对洗脱预柱的溶剂施加电流加热，将其转移至分析柱。该方法可在高效液相色谱集成样品处理后，实现分析物从预柱到分析柱的快速定量转移。
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