Molloy P E, Graham B M, Cupit P M, Grant S D, Porter A J, Cunningham C
Department of Molecular and Cell Biology, Marischal College, University of Aberdeen, Scotland.
Mol Biotechnol. 1995 Dec;4(3):239-45. doi: 10.1007/BF02779017.
This work describes protocols for the production of single-chain antibody and T-cell receptor fragments in E. coli. A choice of methods is given for the purification of the recombinant fragments that rely on the use of either immunoaffinity or metal chelate affinity chromatography. The TCR fragments may have to be denatured and refolded before the fragments attain their proper conformation.
这项工作描述了在大肠杆菌中生产单链抗体和T细胞受体片段的方案。针对重组片段的纯化给出了多种方法选择，这些方法依赖于免疫亲和或金属螯合亲和色谱法的使用。在TCR片段达到其正确构象之前，可能必须对其进行变性和重折叠。
