Pond S M, Gordon R A, Bass L
Department of Medicine, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
Eur J Clin Invest. 1996 Jan;26(1):76-81. doi: 10.1046/j.1365-2362.1996.101246.x.
To determine if the sex differences in clearance of long chain fatty acids by the liver observed in the rat are relevant to humans, the authors isolated hepatocytes from human adult males and females (five per group) and measured the initial (unidirectional) clearance of [3H]-palmitate from buffer containing albumin. The clearance was significantly higher (about twofold) in hepatocytes from females because of a higher permeability of the plasma membrane to the fatty acid. The livers had been perfused with University of Wisconsin (UW) solution and stored for 12-16 h before isolation of the cells. The magnitudes of the clearances in humans were similar to those in the rat when the livers were stored similarly, but lower than in cells isolated from fresh rat liver. When hepatocytes isolated from fresh rat liver were stored in UW solution, clearance of [3H]-palmitate was unaffected. Thus, hepatocytes prepared from intact liver stored for several hours in UW solution do not have as good preservation of function as hepatocytes isolated from fresh liver and then stored in UW solution.
为了确定在大鼠中观察到的肝脏清除长链脂肪酸的性别差异是否与人类相关，作者从成年男性和女性（每组5人）中分离出肝细胞，并测量了含有白蛋白的缓冲液中[3H] - 棕榈酸酯的初始（单向）清除率。由于质膜对脂肪酸的通透性较高，女性肝细胞中的清除率显著更高（约两倍）。在分离细胞之前，肝脏已用威斯康星大学（UW）溶液灌注并储存12 - 16小时。当肝脏以类似方式储存时，人类的清除率大小与大鼠相似，但低于从新鲜大鼠肝脏分离的细胞。当从新鲜大鼠肝脏分离的肝细胞储存在UW溶液中时，[3H] - 棕榈酸酯的清除率不受影响。因此，从完整肝脏在UW溶液中储存数小时制备的肝细胞，其功能保存不如从新鲜肝脏分离后再储存在UW溶液中的肝细胞。
