DiLoreto D, del Cerro C, del Cerro M
Department of Neurobiology, University of Rochester School of Medicine, New York 14642, USA.
Exp Neurol. 1996 Jul;140(1):37-42. doi: 10.1006/exnr.1996.0112.
We have reported that xenografts of human fetal neural retina survive in the subretinal space of cyclosporine-immunosuppressed rats. In view of the current controversy regarding the role of cyclosporine, we wished to determine if cyclosporine immunosuppression was an absolute requirement for retinal xenograft survival. Neural retinas from human fetal eyes obtained within 1 h of termination of pregnancy were stored in Optisol medium (Chiron Vision, Irvine, CA) at 4 degrees C for 2 and 7 days. Retinas were then transplanted to the subretinal space of either cyclosporine-treated (10 mg/kg/day) light-damaged Fischer 344 rat eyes (17 animals, 28 eyes) or to the subretinal space of light-damaged Fischer 344 rat eyes (9 animals, 15 eyes) receiving no cyclosporine treatment. Grafted eyes were observed clinically at 10, 20, and 30 days posttransplantation. At 30 days, the animals were sacrificed and the grafts observed histologically. Human fetal retina xenografted to the subretinal space of immunosuppressed rats survived (9/17 animals, 12/28 eyes), showed good integration with the host retina and initial photoreceptor differentiation. Tissue xenografted to the subretinal space of non-cyclosporine-treated rats was not observed to survive (0/9 animals, 0/15 eyes). A low level cellular reaction was seen around three of the injection sites within the nonimmunosuppressed rats. We conclude that immunosuppression is necessary for the survival of human fetal neural retina xenografted to the subretinal space.
我们曾报道，人胎儿神经视网膜异种移植物可在环孢素免疫抑制大鼠的视网膜下间隙存活。鉴于目前关于环孢素作用的争议，我们希望确定环孢素免疫抑制是否是视网膜异种移植物存活的绝对必要条件。妊娠终止后1小时内获取的人胎儿眼的神经视网膜，在Optisol培养基（Chiron Vision，尔湾，加利福尼亚州）中于4℃保存2天和7天。然后将视网膜移植到接受环孢素治疗（10mg/kg/天）的光损伤Fischer 344大鼠眼的视网膜下间隙（17只动物，28只眼），或移植到未接受环孢素治疗的光损伤Fischer 344大鼠眼的视网膜下间隙（9只动物，15只眼）。移植后10天、20天和30天对移植眼进行临床观察。30天时，处死动物并对移植物进行组织学观察。移植到免疫抑制大鼠视网膜下间隙的人胎儿视网膜存活（9/17只动物，12/28只眼），与宿主视网膜有良好整合并出现初始光感受器分化。移植到未用环孢素治疗大鼠视网膜下间隙的组织未观察到存活（0/9只动物，0/15只眼）。在未免疫抑制大鼠的三个注射部位周围可见低水平细胞反应。我们得出结论，免疫抑制对于移植到视网膜下间隙的人胎儿神经视网膜的存活是必要的。
