Takahashi F, Endo S, Kojima T, Saigo K
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan.
Genes Dev. 1996 Jul 1;10(13):1645-56. doi: 10.1101/gad.10.13.1645.
In Drosophila, Dsrc64 is considered a unique ortholog of the vertebrate c-src; however, we show evidence to the contrary. The closest relative of vertebrate c-src so far found in Drosophila is not Dsrc64, but Dsrc41, a gene identified for the first time here. In contrast to Dsrc64, overexpression of wild-type Dsrc41 caused little or no appreciable phenotypic change in Drosophila. Both gain-of-function and dominant-negative mutations of Dsrc41 caused the formation of supernumerary R7-type neurons, suppressible by one-dose reduction of boss, sev, Ras1, or other genes involved in the Sev pathway. Dominant-negative mutant phenotypes were suppressed and enhanced, respectively, by increasing and decreasing the copy number of wild-type Dsrc41. Colocalization of Dsrc41 protein, actin fibers and DE-cadherin, and Dsrc41-dependent disorganization of actin fibers and putative adherens junctions in precluster cells suggested that Dsrc41 may be involved in the regulation of cytoskeleton organization and cell-cell contacts in developing ommatidia.
在果蝇中，Dsrc64被认为是脊椎动物c-src的独特直系同源基因；然而，我们展示了相反的证据。目前在果蝇中发现的与脊椎动物c-src最接近的亲属不是Dsrc64，而是Dsrc41，这是一个在此首次鉴定的基因。与Dsrc64相反，野生型Dsrc41的过表达在果蝇中几乎没有引起或没有引起明显的表型变化。Dsrc41的功能获得性突变和显性负性突变都导致了额外的R7型神经元的形成，通过减少boss、sev、Ras1或Sev途径中的其他基因的剂量可以抑制这种现象。通过增加和减少野生型Dsrc41的拷贝数，显性负性突变体的表型分别受到抑制和增强。Dsrc41蛋白、肌动蛋白纤维和DE-钙黏蛋白的共定位，以及Dsrc41依赖的前簇细胞中肌动蛋白纤维和假定的黏着连接的紊乱，表明Dsrc41可能参与了发育中的小眼的细胞骨架组织和细胞间接触的调节。
