包含与人类染色体17q同源的小鼠染色体11（MMU11）部分的体细胞杂种的衍生与表征。

Derivation and characterization of a somatic cell hybrid containing the portion of mouse chromosome 11 (MMU11) homologous to human chromosome 17q.

作者信息

Morris-Rosendahl D J, Schriever-Schwemmer G, Robinson T J

机构信息

Department of Zoology and Entomology, University of Pretoria, South Africa.

出版信息

J Hered. 1996 May-Jun;87(3):252-8. doi: 10.1093/oxfordjournals.jhered.a022997.

DOI:10.1093/oxfordjournals.jhered.a022997

PMID:8683100

Abstract

To contribute to the physical gene map of mouse chromosome 11 (MMU11) and to extend the mapping resources available for this chromosome, we have produced mouse x rat somatic cell hybrids containing only bands B5 to E of MMU11. Characterization of the hybrids by polymerase chain reaction (PCR) amplification and Southern blot analyses of MMU11 markers revealed two hybrids, T16Ad14B and T16Ad19A, that had selectively retained the 3(11) translocation product containing distal MMU11 (bands B5-E). Cytogenetic analysis of the hybrid T16Ad14B by fluorescence in situ hybridization (FISH) and conventional G-banding confirmed the presence of the 3(11) translocation chromosome. Mapping of markers in both the T16Ad14B and T16Ad19A hybrids localized the T16Ad translocation breakpoint between the proximal markers Atplb2 and Acrb and the more distal markers Scya2 and Mpo. Loci for D11Mit5, Rpo2-1, Trp53, Glut4, Acrb, and Atplb2 could all be localized proximal to the T16Ad breakpoint in band B5, between bands B1 and B5 on MMU11.

摘要

为构建小鼠11号染色体（MMU11）的物理基因图谱并扩展该染色体可用的定位资源，我们制备了仅包含MMU11的B5至E带的小鼠×大鼠体细胞杂种。通过聚合酶链反应（PCR）扩增和MMU11标记的Southern印迹分析对杂种进行表征，发现两个杂种T16Ad14B和T16Ad19A选择性地保留了包含MMU11远端（B5 - E带）的3(11)易位产物。通过荧光原位杂交（FISH）和传统G显带对杂种T16Ad14B进行细胞遗传学分析，证实了3(11)易位染色体的存在。对T16Ad14B和T16Ad19A杂种中的标记进行定位，将T16Ad易位断点定位在近端标记Atplb2和Acrb与更远端标记Scya2和Mpo之间。D11Mit5、Rpo2 - 1、Trp53、Glut4、Acrb和Atplb2的基因座都可以定位在MMU11上B1和B5带之间B5带中T16Ad断点的近端。