Contreras-Brodin B, Karlsson A, Nilsson T, Rymo L, Klein G
Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
J Gen Virol. 1996 Jun;77 ( Pt 6):1159-62. doi: 10.1099/0022-1317-77-6-1159.
We have studied the activity of reporter plasmids, carrying the Epstein-Barr virus (EBV) nuclear antigen (EBNA) promoters Wp and Cp, in a group of somatic cell hybrids obtained by fusing EBV-positive lymphoblastoid cell lines or group II/III Burkitt's lymphoma cell lines with non-B cell lines. In B/non-B cell hybrids of this type, B cell markers are extinguished as a rule, in parallel with the inactivation of Cp or Wp and down-regulation of EBNA-2-6 expression. A Wp-carrying reporter construct was active in non-B cell lines. Only cells with a B cell phenotype could support the activity of Cp-carrying plasmids. EBNA-2 transactivated Cp only in B cells. Our data suggest that while Wp can be used for EBNA transcription in B and non-B cells, Cp activity is restricted to B cells. The inability of EBNA-2 to transactivate Cp in non-B cells indicates that other factors present in B cells might be involved in Cp transactivation.
我们研究了携带爱泼斯坦-巴尔病毒（EBV）核抗原（EBNA）启动子Wp和Cp的报告质粒在一组体细胞杂种中的活性，这些体细胞杂种是通过将EBV阳性淋巴母细胞系或II/III组伯基特淋巴瘤细胞系与非B细胞系融合而获得的。在这种类型的B/非B细胞杂种中，B细胞标志物通常会消失，同时Cp或Wp失活以及EBNA-2-6表达下调。携带Wp的报告构建体在非B细胞系中具有活性。只有具有B细胞表型的细胞才能支持携带Cp质粒的活性。EBNA-2仅在B细胞中反式激活Cp。我们的数据表明，虽然Wp可用于B细胞和非B细胞中的EBNA转录，但Cp的活性仅限于B细胞。EBNA-2在非B细胞中无法反式激活Cp，这表明B细胞中存在的其他因素可能参与Cp的反式激活。
