Touitou R, Cochet C, Joab I
CNRS URA 1301, Institut Gustave Roussy, Villejuif, France.
J Gen Virol. 1996 Jun;77 ( Pt 6):1163-8. doi: 10.1099/0022-1317-77-6-1163.
The Epstein-Barr virus (EBV) open reading frame (ORF) BCRF1, expressed in the late phase of the viral cycle, encodes a homologue of human interleukin-10 (hIL-10). Unspliced, 3' co-terminal transcripts of 0.8 and 1.6 kb from O-tetradecanoylphorbol 13-acetate (TPA)-treated B95-8 cells have been described but other results indicated the existence of uncharacterized transcript(s) initiated upstream of the 1.6 kb BCRF1 mRNA. Here we describe two additional large transcripts of the BCRF1 ORF, a possibly spliced product of 3.5 kb and an unspliced product of 4.5 kb. The time course of the expression of BCRF1 transcripts and of the secreted protein from Akata cells were also determined.
爱泼斯坦-巴尔病毒（EBV）开放阅读框（ORF）BCRF1在病毒周期后期表达，编码人白细胞介素-10（hIL-10）的同源物。已描述了来自经十四烷酰佛波醇-13-乙酸酯（TPA）处理的B95-8细胞的0.8和1.6 kb未剪接、3' 共末端转录本，但其他结果表明存在于1.6 kb BCRF1 mRNA上游起始的未表征转录本。在此，我们描述了BCRF1 ORF的另外两个大转录本，一个可能剪接的3.5 kb产物和一个未剪接的4.5 kb产物。还确定了Akata细胞中BCRF1转录本和分泌蛋白表达的时间进程。
