Junge W D, Rätz K H, Clasen E, Scholz K, Neumann G
Zentralbl Gynakol. 1977;99(7):435-40.
The authors report the development and investigation of a new technique for freezing and storage of human spermatozoa. Utilizing isolated container of different kind the average rate of freezing was between 1,3 grd/min and 14 grd/min in the sphere from 5 degrees C to--10 degrees C and between 2,9 grd/min to 55 grd/min in the sphere from--10 degrees C to--60 degrees C. The technical expense was low. Semen specime were provided by 33 andrological patients. A total of 53 samples of human semen were frozen and stored for periods of time up to 25 days. The authors used the cryoprotective medium from Behrman and Sawada. The best results were obtained in the mode of freezing at 2,8 grd/min respectively 12 grd/min in ejaculates of original motility about 80%. The recovery of motility and fertilizability of spermatozoa was 50% of original motility, in cases of oligospermia only 34%. The results of this new technique of freezing and storage of human spermatozoa are similar to other new reports in literature.
作者报告了一种用于人类精子冷冻和储存的新技术的开发与研究。使用不同类型的隔离容器，在5摄氏度至 -10摄氏度范围内，冷冻平均速率在1.3摄氏度/分钟至14摄氏度/分钟之间，在 -10摄氏度至 -60摄氏度范围内，冷冻平均速率在2.9摄氏度/分钟至55摄氏度/分钟之间。技术成本较低。精液样本由33名男科患者提供。总共53份人类精液样本被冷冻并储存长达25天。作者使用了贝尔曼和泽田的冷冻保护介质。在原始活力约为80%的射精样本中，分别以2.8摄氏度/分钟和12摄氏度/分钟的冷冻模式获得了最佳结果。精子活力和受精能力的恢复率为原始活力的50%，少精子症患者的恢复率仅为34%。这种人类精子冷冻和储存新技术的结果与文献中的其他新报告相似。
