Dmitrieva L N, Shemanova G F, Vlasova E V, Akhundova K A
Zh Mikrobiol Epidemiol Immunobiol. 1977 Apr(4):110-4.
The authors present the results of comparative study of the properties of experimental perfringens toxoids obtained from purified alpha-toxoids of different degrees of purity. Experimental toxoids proved to possess a greater immunogenicity than preparations obtained by detoxication of alpha-toxin under conditions of cultural fluid, the greater--the more the purity of alpha-toxin used for procuring the experimental toxoid. C1. perfringens alpha-toxin recovered as a result of two-stage alpha-toxin purification, including its primary concentration and fractionation on DEAE-cellulose under conditions of negative alpha-toxin sorption, changed during detoxication into toxoid whose immunogenicity exceeded that of manufactured preparations 3--4 fold. The toxoid was harmless and sorbed in a dose of 100 BU on 2--3 mg of aluminium hydroxide; stability of its antigenic properties and its yield was not less than those of manufactured toxoids. Perfringens toxoid prepared from highly purified alpha-toxoid was 10 times greater by immunogenicity than the manufactured preparation, and was sorbed on 1--2 mg of aluminium hydroxide.
