兔肠上皮细胞的DNA聚合酶γ
DNA polymerase gamma of rabbit intestinal epithelial cells.
作者信息
Kamać J, Siedlecki J A, Zmudzka B
出版信息
Acta Biochim Pol. 1977;24(1):65-74.
PMID:868438
Abstract
- DNA-directed DNA polymerase gamma was isolated from epithelial cells of the rabbit small intestine, and characterized. 2. The molecular weight of the enzyme, determined by Sephadex G-200 filtration was 105 000 +/- 15%. 3. The enzyme showed preference for poly(A) replication on poly(A)-poly(dT) or poly(A)-oligo(dT) 12-18 templates, as compared with activated DNA. Poly(C)-oligo(dG)12-18 replication was not observed. 4. The Michaelis constants for dTTP in replication of activated DNA and poly(A)-oligo(dT)12-18 were 3.3 and 2.0 micron, respectively.
摘要
- 从兔小肠上皮细胞中分离并鉴定了DNA指导的DNA聚合酶γ。2. 通过Sephadex G - 200过滤测定，该酶的分子量为105000±15%。3. 与活化DNA相比，该酶在聚（A）-聚（dT）或聚（A）-寡聚（dT）12 - 18模板上对聚（A）复制表现出偏好。未观察到聚（C）-寡聚（dG）12 - 18复制。4. 在活化DNA和聚（A）-寡聚（dT）12 - 18复制中，dTTP的米氏常数分别为3.3和2.0微摩尔。
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