Bogers J J, Nibbeling H A, Deelder A M, van Marck E A
Laboratory of Pathology, University of Antwerp (UIA), Wilrijk, Belgium.
Parasitology. 1996 Jun;112 ( Pt 6):537-43. doi: 10.1017/s0031182000066117.
The detection of egg-derived antigens in the serum and urine of Schistosoma mansoni-infected individuals and experimental animals would provide an alternative method to assess the tissue egg burden. The detected levels are, however, not only a function of the amounts of antigen produced, but also of the processing or clearance by the host. In the present study the immunolocalization pattern of antigens using 2 recently described monoclonal antibodies to repetitive carbohydrate epitopes of S. mansoni soluble egg antigen (114-5B1-A and 114-4D12-A) in various organs of the host was investigated. In the liver strong immunoreactivity could be detected around the entrapped eggs and in egg-shells, as well as in Kupffer cells accumulating both antigen and schistosomal pigment. In the spleen, immunohistochemistry revealed antigen in the plasma as well as in secondary lysosomes of macrophages. Strong labelling was found in the vesicles of the eosinophilic granulocytes: indirect evidence perhaps for the presence of antigen-antibody complexes. In conclusion, the secreted egg antigens were sequestered in the reticulo-endothelial macrophages of the liver and the spleen as already partly described for worm-derived antigens. The presence of large quantities of antigenic material in the spleen could suggest an important role of this organ in the clearance of antigen and might even provide an additional explanation for the hepatosplenomegaly mainly present in S. mansoni-infected children.
在曼氏血吸虫感染个体和实验动物的血清及尿液中检测源自虫卵的抗原，将为评估组织虫卵负荷提供一种替代方法。然而，检测到的水平不仅取决于产生的抗原量，还取决于宿主的处理或清除情况。在本研究中，使用最近描述的两种针对曼氏血吸虫可溶性虫卵抗原重复碳水化合物表位的单克隆抗体（114 - 5B1 - A和114 - 4D12 - A），研究了抗原在宿主各器官中的免疫定位模式。在肝脏中，在被困虫卵周围、卵壳以及积聚抗原和血吸虫色素的库普弗细胞中可检测到强免疫反应性。在脾脏中，免疫组织化学显示血浆以及巨噬细胞的次级溶酶体中有抗原。在嗜酸性粒细胞的囊泡中发现强标记：这可能间接证明了抗原 - 抗体复合物的存在。总之，分泌的虫卵抗原被隔离在肝脏和脾脏的网状内皮巨噬细胞中，这在一定程度上已针对源自虫体的抗原进行过描述。脾脏中存在大量抗原物质可能表明该器官在抗原清除中起重要作用，甚至可能为主要在曼氏血吸虫感染儿童中出现的肝脾肿大提供额外解释。
