Bogers J, Jacobs W, Segers K, Van Daele A, Weyler J, Van Marck E
Department of Pathology, University of Antwerp (UIA), Belgium.
Pathol Res Pract. 1996 Jan;192(1):10-4. doi: 10.1016/S0344-0338(96)80123-3.
The differential diagnosis between malignant mesothelioma and benign pleural hyperplasia constitutes a well-known problem. In the present study we examined unbiased stereological techniques to assess the mean nuclear volume (MNV) using the point-sampled intercepts (PSI) in 37 cases of malignant mesothelioma and in 28 cases of benign pleural hyperplasia. Neither the use of different fixatives nor the histological type of malignant mesothelioma produced any significant difference on the measured nuclear volume. The differences observed between the MNV data obtained from benign pleural hyperplasia and those from any of the three types of malignant mesothelioma were found to be highly significant. All lesions with an MNV larger than 250 microns3 were found in our study to correspond to the malignant mesothelioma type, while an MNV that was smaller than 200 microns3 could only be detected in benign specimens. These observations lead us to propose the MNV measurement using PSI as an additional tool to enhance the differential diagnosis of malignant mesothelioma versus benign pleural hyperplasia.
恶性间皮瘤与良性胸膜增生之间的鉴别诊断是一个众所周知的问题。在本研究中，我们运用无偏倚的体视学技术，通过点采样截距法（PSI）测定了37例恶性间皮瘤和28例良性胸膜增生病例的平均核体积（MNV）。无论是使用不同的固定剂，还是恶性间皮瘤的组织学类型，均未对所测核体积产生任何显著差异。结果发现，良性胸膜增生所获MNV数据与三种类型恶性间皮瘤中任何一种的MNV数据之间的差异具有高度显著性。在我们的研究中，所有平均核体积大于250立方微米的病变均为恶性间皮瘤类型，而平均核体积小于200立方微米的情况仅在良性标本中检测到。这些观察结果使我们建议将运用点采样截距法测定平均核体积作为一种辅助手段，以加强恶性间皮瘤与良性胸膜增生的鉴别诊断。
