Zhao B Y, Collins M T, Czuprynski C J
University of Wisconsin-Madison 53706, USA.
Res Vet Sci. 1996 Mar;60(2):190-2. doi: 10.1016/s0034-5288(96)90019-3.
Bovine monocytes freshly isolated from peripheral blood were induced to produce nitric oxide by exposing them to recombinant bovine interferon gamma (rbIFN-y) and Escherichia coli lipopolysaccharide (LPS) in vitro. Moderate amounts of nitric oxide were induced by rbIFN-gamma alone, but larger amounts were induced by rbIFN-gamma and LPS together, the amount being dependent on the quantity of rbIFN-gamma added. Reactive nitrogen intermediates (RNI) were produced within six hours, their concentration peaked at four days and they were detectable for at least eight days after the cells had been stimulated with rbIFN-gamma and LPS. The production of RNI was diminished by the addition of NG-monomethyl-L-arginine. The data suggest that bovine monocytes can produce RNI via a pathway involving an inducible nitric oxide synthase.
从外周血中新鲜分离的牛单核细胞，在体外通过暴露于重组牛干扰素γ（rbIFN-γ）和大肠杆菌脂多糖（LPS）来诱导产生一氧化氮。单独的rbIFN-γ可诱导产生适量的一氧化氮，但rbIFN-γ和LPS共同作用时可诱导产生更多的一氧化氮，其产生量取决于所添加的rbIFN-γ的量。活性氮中间体（RNI）在6小时内产生，其浓度在4天时达到峰值，并且在用rbIFN-γ和LPS刺激细胞后至少8天内均可检测到。添加NG-甲基-L-精氨酸可减少RNI的产生。数据表明，牛单核细胞可通过涉及诱导型一氧化氮合酶的途径产生RNI。
