Schlaifer D, Krajewski S, Galoin S, Rigal-Huguet F, Laurent G, Massip P, Pris J, Delsol G, Reed J C, Brousset P
Service d'Hématologie, Centre Hospitalier Universitaire, Hôpital de Purpan, Toulouse, France.
Am J Pathol. 1996 Jul;149(1):177-85.
The expression of the apoptosis-regulating genes Bcl-2, Bcl-x, Bax, Mcl-1, and p53 analyzed in 4 cases of human immunodeficiency virus (HIV)-associated Hodgkin's disease, in 36 cases of HIV-related non-Hodgkin's lymphomas (NHLs), and in 109 cases of non-HIV-related NHLs by using immunohistochemistry. HIV-associated Hodgkin's disease samples were positive for all markers. For the HIV-related NHL samples, 36, 66, 88, 100, and 94% of the cases were Bcl-2, Bcl-x, Bax, Mcl-1, and p53 were found to be expressed in 69, 65, 82, 83, and 42%, respectively. No significant differences were observed in Bax and Mcl-1 staining between HIV-unrelated NHLs of B cell and T cell types. In contrast, Bcl-2 was positive in 66/79 (83%) and 10/30 (33%) of B cell and T cell HIV-unrelated NHLs, respectively (P2 < 0.001). Peculiar patterns were observed for hairy cell leukemia (Bax+, Bcl-2+, Mcl-1-) and for anaplastic large cell lymphoma (Bax+, Mcl-1+, Bcl-2-) in HIV-unrelated NHLs. Of interest, all cases with a positive expression of Bax were also found to express either Mcl-1 and/or Bcl-2, suggesting that Mcl-1 and Bcl-2 may counteract the pro-apoptosis function of Bax in vivo by protein-protein interaction within the tumor cell, as demonstrated previously in vitro. These results suggest that apoptosis regulation may have a role in the pathogenesis of some HIV-related and HIV-unrelated NHLs.
通过免疫组织化学方法，分析了4例人类免疫缺陷病毒（HIV）相关霍奇金淋巴瘤、36例HIV相关非霍奇金淋巴瘤（NHL）以及109例非HIV相关NHL中凋亡调节基因Bcl-2、Bcl-x、Bax、Mcl-1和p53的表达情况。HIV相关霍奇金淋巴瘤样本的所有标志物均呈阳性。对于HIV相关NHL样本，分别有36%、66%、88%、100%和94%的病例Bcl-2、Bcl-x、Bax、Mcl-1和p53呈阳性表达；在非HIV相关的B细胞和T细胞型NHL中，分别有69%、65%、82%、83%和42%的病例表达这些基因。在B细胞和T细胞型非HIV相关NHL之间观察到，Bax和Mcl-1染色无显著差异。相比之下，Bcl-2在B细胞型非HIV相关NHL的66/79（83%）病例和T细胞型非HIV相关NHL的10/30（33%）病例中呈阳性（P2<0.001）。在非HIV相关NHL中，毛细胞白血病（Bax+、Bcl-2+、Mcl-1-）和间变性大细胞淋巴瘤（Bax+、Mcl-1+、Bcl-2-）呈现出特殊的表达模式。有趣的是，所有Bax阳性表达的病例也被发现表达Mcl-1和/或Bcl-2，这表明Mcl-1和Bcl-2可能通过肿瘤细胞内的蛋白质-蛋白质相互作用在体内抵消Bax的促凋亡功能，正如先前在体外所证明的那样。这些结果表明，凋亡调节可能在一些HIV相关和非HIV相关NHL的发病机制中起作用。
