Miau L H, Jan Y W, Shen B J, Tsai W H, Lee H S, Lee S C
Institute of Biochemical Sciences, College of Science, National Taiwan University, Taipei, Taiwan.
Biochem Biophys Res Commun. 1996 Jun 25;223(3):487-91. doi: 10.1006/bbrc.1996.0921.
Stromal cells can interact with parenchymal cells by secreting various cytokines to affect the growth, differentiation or movement of the latter. Here we report the identification and characterization of a novel variant hepatocyte growth factor (HGF) from the conditioned medium of stromal cells derived from mouse spleen. Compared to human HGF, it has much lower heparin-binding activity and lacks the beta-chain. Its molecular weight, 70 kDa, is very close to that of the alpha-chain of HGF. Human HGF homologue was not found in the conditioned medium. The conditioned medium of stromal cells, like recombinant HGF, could inhibit the growth of rat hepatoma cells. The inhibitory activity was presumably attributed to this novel HGF because the inhibitory activity, as the existence of this novel HGF, was confined to the identical fractions after heparin-column chromatography. Furthermore, this activity could be specifically abrogated by neutralizing anti-HGF antibodies.
基质细胞可通过分泌多种细胞因子与实质细胞相互作用，从而影响后者的生长、分化或运动。在此，我们报告从小鼠脾脏来源的基质细胞条件培养基中鉴定并表征了一种新型变体肝细胞生长因子（HGF）。与人类HGF相比，它具有低得多的肝素结合活性且缺乏β链。其分子量为70 kDa，与HGF的α链非常接近。在条件培养基中未发现人类HGF同源物。基质细胞的条件培养基与重组HGF一样，可抑制大鼠肝癌细胞的生长。这种抑制活性可能归因于这种新型HGF，因为该抑制活性与这种新型HGF的存在一样，在肝素柱层析后局限于相同的组分中。此外，这种活性可被中和抗HGF抗体特异性消除。
