Sill H, Goldman J M, Cross N C
LRF Centre for Adult Leukaemia, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Br J Cancer. 1996 Jul;74(2):255-7. doi: 10.1038/bjc.1996.347.
We have analysed samples from 20 patients with acute myeloid leukaemia for microsatellite alterations by comparing constitutional DNA and DNA from leukaemic samples. Twelve microsatellites were amplified by PCR and investigated for novel bands, indicative of microsatellite instability, or for loss of heterozygosity. Out of 215 paired amplifications, no additional bands were observed at any locus in any of the samples analysed and loss of heterozygosity was found only as four loci from three patients. These results suggest that microsatellite alterations are very uncommon in acute myeloid leukaemia.
我们通过比较白血病患者的基因组DNA和白血病样本中的DNA，分析了20例急性髓系白血病患者样本的微卫星改变情况。通过聚合酶链反应（PCR）扩增了12个微卫星，并检测是否存在新条带（指示微卫星不稳定性）或杂合性缺失。在215次配对扩增中，分析的任何样本的任何位点均未观察到额外条带，仅在3例患者的4个位点发现了杂合性缺失。这些结果表明，微卫星改变在急性髓系白血病中非常罕见。
