Cloning and sequencing of cDNAs encoding the human sperm protein, Sp17.

作者信息

Lea I A, Richardson R T, Widgren E E, O'Rand M G

机构信息

Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599, USA.

出版信息

Biochim Biophys Acta. 1996 Jul 17;1307(3):263-6. doi: 10.1016/0167-4781(96)00077-2.

In the present study we have cloned and sequenced two testis-specific cDNAs (1.3 kb and 1.6 kb) encoding a human sperm protein, designated HSp17. Each cDNA gave rise to identical protein sequences and differed only in the 5' untranslated region. The predicted amino-acid sequence revealed a protein of 17.5 kDa which exhibited a high degree of homology with both rabbit and mouse Sp17. Analysis of native and recombinant Sp17 by SDS-PAGE has shown the apparent molecular weight of the protein to be 24.5 kDa.