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整合素与具有4个跨膜结构域的蛋白质（TM4蛋白）在细胞表面形成的新型复合物的特性分析。

Characterization of novel complexes on the cell surface between integrins and proteins with 4 transmembrane domains (TM4 proteins).

作者信息

Berditchevski F, Zutter M M, Hemler M E

机构信息

Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.

出版信息

Mol Biol Cell. 1996 Feb;7(2):193-207. doi: 10.1091/mbc.7.2.193.

DOI:10.1091/mbc.7.2.193
PMID:8688552
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC275873/
Abstract

Here we identified several new integrin/TM4 protein complexes on the cell surface. By immunoprecipitation using nonstringent conditions, and by reciprocal immunoprecipitation, we found that alpha 3 beta 1 and alpha 6 beta 1 integrins but not alpha 2 beta 1, alpha 5 beta 1, or alpha 6 beta 4 integrins associated with CD9 and CD81 in alpha 3 beta 1/CD81, alpha 3 beta 1/CD9, alpha 6 beta 1/CD81, and alpha 6 beta 1/CD9 complexes. Also, cross-linking experiments established that alpha 3 beta 1/CD81, alpha 3 beta 1/CD9, and alpha 3 beta 1/CD63 associations occur on the surface of intact cells and suggested that a critical interaction site is located within extracellular domains. Cross-linking in conjunction with reimmunoprecipitation indicated that larger multi-component alpha 3 beta 1/TM4/TM4 complexes (alpha 3 beta 1/CD9/CD63, alpha 3 beta 1/CD81/CD63, and alpha 3 beta 1/CD9/CD81) also could be detected on the cell surface. Immunofluorescent staining showed redistribution of alpha 3 beta 1/TM4 complexes toward the periphery of cells plated on various extracellular matrix substrates and also showed that these complexes were localized in cell footprints. Staining of human tissues yielded additional results consistent with co-localization of alpha 3 beta 1 and CD9, CD63, and CD81 proteins. In conclusion we suggest that the prevalence of integrin/TM4 complexes in diverse cellular environments is indicative of their general physiological importance.

摘要

在此，我们在细胞表面鉴定出了几种新的整合素/TM4蛋白复合物。通过使用非严格条件的免疫沉淀以及反向免疫沉淀，我们发现α3β1和α6β1整合素而非α2β1、α5β1或α6β4整合素，在α3β1/CD81、α3β1/CD9、α6β1/CD81和α6β1/CD9复合物中与CD9和CD81相关联。此外，交联实验证实α3β1/CD81、α3β1/CD9和α3β1/CD63的结合发生在完整细胞表面，并表明关键的相互作用位点位于细胞外结构域内。交联结合再免疫沉淀表明，在细胞表面也能检测到更大的多组分α3β1/TM4/TM4复合物（α3β1/CD9/CD63、α3β1/CD81/CD63和α3β1/CD9/CD81）。免疫荧光染色显示α3β1/TM4复合物向铺在各种细胞外基质底物上的细胞周边重新分布，还表明这些复合物定位于细胞足迹中。人体组织染色产生了与α3β1和CD9、CD63及CD81蛋白共定位一致的其他结果。总之，我们认为整合素/TM4复合物在不同细胞环境中的普遍存在表明了它们一般的生理重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/5e0ee7bb1d6b/mbc00009-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/c7bdffca9f9d/mbc00009-0011-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/4a76d87185d3/mbc00009-0012-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/fb9a0657a6c6/mbc00009-0013-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/21572ba4f74d/mbc00009-0013-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/730426153017/mbc00009-0014-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/ce04ebd5aa86/mbc00009-0015-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/9445806897a7/mbc00009-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/1b9ff8162572/mbc00009-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/5facd78752d5/mbc00009-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/5e0ee7bb1d6b/mbc00009-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/c7bdffca9f9d/mbc00009-0011-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/4a76d87185d3/mbc00009-0012-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/fb9a0657a6c6/mbc00009-0013-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/21572ba4f74d/mbc00009-0013-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/730426153017/mbc00009-0014-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/ce04ebd5aa86/mbc00009-0015-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/9445806897a7/mbc00009-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/1b9ff8162572/mbc00009-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/5facd78752d5/mbc00009-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a66/275873/5e0ee7bb1d6b/mbc00009-0020-a.jpg

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