Heath D D, Hatcher D R, Hilbish T J
Department of Biological Sciences, University of South Carolina, Columbia 29208, USA.
Mol Ecol. 1996 Jun;5(3):443-7.
M. californianus is the dominant marine mussel species on exposed rocky shores, while M. trossulus is usually the dominant mussel species in more sheltered waters on the west coast of North America. Since these species are physical indistinguishable when small (< 10.0 mm), we developed two polymerase chain reaction (PCR) -based markers to discriminate between them. Using these markers, we identified mussels taken from an exposed coast (n = 114), a sheltered harbour (n = 80), and an upper-intertidal pool (n = 42) on the west coast of Vancouver Island, British Columbia, Canada. M. californianus were found only on the open coast. Small M. trossulus (< 20.0 mm) were common to all three sample sites, but were extremely rare at larger sizes (> 20.0 mm) on the open coast. Our results indicate that M. californianus are excluded from sheltered waters via early life factors, while M. trossulus are excluded from the open coast due to mortality later in life.
加州贻贝是暴露岩石海岸上占主导地位的海洋贻贝物种，而多纹贻贝通常是北美西海岸更隐蔽水域中的优势贻贝物种。由于这些物种在幼小时（<10.0毫米）在形态上难以区分，我们开发了两种基于聚合酶链反应（PCR）的标记来区分它们。利用这些标记，我们鉴定了从加拿大不列颠哥伦比亚省温哥华岛西海岸的一个暴露海岸（n = 114）、一个隐蔽港口（n = 80）和一个潮间带上部水池（n = 42）采集的贻贝。仅在开阔海岸发现了加州贻贝。小型多纹贻贝（<20.0毫米）在所有三个采样点都很常见，但在开阔海岸较大尺寸（>20.0毫米）时极为罕见。我们的结果表明，加州贻贝因早期生活因素被排除在隐蔽水域之外，而多纹贻贝则因后期生活中的死亡率而被排除在开阔海岸之外。
