大鼠8号染色体上稀释性角弓反张（dop）基因的定位

Mapping of the dilute-opisthotonus (dop) gene on chromosome 8 of the rat.

作者信息

Ohno K, Kanou Y, Oda S, Wakasugi N, Inouye M, Yamamura H

机构信息

School of Agricultural Sciences, Nagoya University, Japan.

出版信息

Exp Anim. 1996 Jan;45(1):71-5. doi: 10.1538/expanim.45.71.

DOI:10.1538/expanim.45.71

PMID:8689583

Abstract

The rat dilute-opisthotonus (dop) autosomal recessive gene, causing ataxia and coat color dilution, was mapped on chromosome 8 by PCR-amplified microsatellite markers. To facilitate the linkage analysis, an intersubspecific cross with a Japanese wild rat strain was used. The recombination frequencies were 12.8% between Apoc3 and dop, and 32.1% between dop and Mylc1v. The following order of three genes is proposed; Apoc3-dop-Mylc1v. This mutation appears to be homologous to dilute-lethal (d1) of the mouse in terms of clinical symptoms, coat color effect and chromosomal location of the gene loci. Key words: ataxic mutant rat, dilute-opisthotonus (dop), gene mapping.

摘要

大鼠隐性稀毛后弓反张（dop）常染色体基因可导致共济失调和被毛颜色变浅，通过聚合酶链反应（PCR）扩增的微卫星标记将其定位在8号染色体上。为便于连锁分析，采用了与日本野生大鼠品系的种间杂交。载脂蛋白C3（Apoc3）与dop之间的重组频率为12.8%，dop与肌球蛋白轻链1v（Mylc1v）之间的重组频率为32.1%。提出了三个基因的以下顺序：Apoc3 - dop - Mylc1v。就临床症状、被毛颜色效应和基因座的染色体定位而言，该突变似乎与小鼠的稀毛致死（d1）同源。关键词：共济失调突变大鼠，隐性稀毛后弓反张（dop），基因定位