Kawamura M, Washizu T, Ishida T, Tomoda I
Department of Clinical Pathology, Nippon Veterinary and Animal Science University, Tokyo, Japan.
Exp Anim. 1996 Jan;45(1):85-8. doi: 10.1538/expanim.45.85.
Biopsied canine liver tissues were investigated histochemically by electron microscopy to demonstrate copper localization. The samples were fixed and stained with the modified sulfide-silver method with a combination of iron and zinc removal. Using this method, the hepatic copper, even at a low concentration of 30 micrograms/g dry weight, was detectable. Copper was found in the hepatocyte lysosomes or in the cytoplasm as electrondense granules.
通过电子显微镜对活检的犬肝脏组织进行组织化学研究，以显示铜的定位。样品经过固定，并用改良的硫化银法染色，同时去除铁和锌。使用这种方法，即使肝铜浓度低至30微克/克干重也可检测到。在肝细胞溶酶体或细胞质中发现铜以电子致密颗粒的形式存在。
