Kikuchi H, Sawada T, Yanagisawa T
Department of Dental Pharmacology, School of Dentistry, Showa University, Tokyo, Japan.
Bull Tokyo Dent Coll. 1994 Nov;35(4):189-96.
The dental papillae isolated from rat incisor were examined morphologically and autoradiographically in order to investigate progressive odontoblast differentiation in vitro. Four to eight days after incubation, the preodontoblasts which were labelled with 3H-thymidine became elongated, polarized, odontoblast-like cells with long cytoplasmic processes. They produced an 3H-proline labelled extracellular matrix on the substrate with which the dental papillae were coated. In addition, the odontoblasts located on the initially formed predentin maintained their ordinary shape as elongated cell bodies with long cytoplasmic processes and polarity fo the organelles during the term of culture. The results indicate that the surface of the agar gel substrate may be a critical requirement for odontoblast differentiation, development, and maturation in the absence of both the basement membrane and the enamel epithelium in the culture system used in this study.
为了研究体外成牙本质细胞的渐进性分化，对从大鼠切牙分离出的牙乳头进行了形态学和放射自显影检查。培养4至8天后，用3H-胸腺嘧啶核苷标记的前成牙本质细胞变成了具有长细胞质突起的细长、极化的成牙本质样细胞。它们在包被牙乳头的底物上产生了3H-脯氨酸标记的细胞外基质。此外，位于最初形成的前期牙本质上的成牙本质细胞在培养期间保持其普通形状，即具有长细胞质突起的细长细胞体和细胞器的极性。结果表明，在本研究使用的培养系统中，在没有基底膜和釉质上皮的情况下，琼脂凝胶底物表面可能是成牙本质细胞分化、发育和成熟的关键条件。
