Pironcheva G L, Miteva K, Vaisberg C, Russev G
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Cytobios. 1995;83(335):207-10.
The effect of freezing at different temperatures, -20 degrees C, -70 degrees C and -196 degrees C, on the transcription activity of buffalo sperm chromatin was investigated. The kinetic data of incorporation of 3H-UTP in pre-mRNA indicate that temperatures of -70 degrees C and -196 degrees C are the most favourable for preservation, since transcription activity gradually increases with time, reaching maximum values within 30 min. Freezing at -20 degrees C or preservation at 4 degrees C leads to a rapid decrease of transcriptional activity within 10 and 20 min, respectively. It is suggested that the optimal temperature for storage of buffalo spermatozoa is below -20 degrees C as judged by the transcription assay.
研究了在不同温度（-20℃、-70℃和-196℃）下冷冻对水牛精子染色质转录活性的影响。3H-UTP掺入前体mRNA的动力学数据表明，-70℃和-196℃的温度最有利于保存，因为转录活性随时间逐渐增加，在30分钟内达到最大值。在-20℃冷冻或在4℃保存分别导致转录活性在10分钟和20分钟内迅速下降。根据转录试验判断，水牛精子储存的最佳温度低于-20℃。
