Romanska H M, Bishop A E, Lee J C, Walsh F S, Spitz L, Polak J M
Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Dig Dis Sci. 1996 Jul;41(7):1298-302. doi: 10.1007/BF02088550.
Our previous study of neural cell adhesion molecule (NCAM) in childhood Hirschsprung's disease demonstrated increased expression on intestinal muscle, especially muscularis mucosae, in aganglionic bowel. The present study was undertaken to test whether this increased NCAM expression is a feature of congenital aganglionosis rather than being a nonspecific feature of constipation. We studied specimens from six patients (20-60 years old; five women and one man) operated on for idiopathic long-standing constipation, using immunocytochemistry for NCAM and protein gene product 9.5 (PGP 9.5). Results showed that in adult constipated bowel the pattern of NCAM expression is similar to that seen in controls. There was no expression of NCAM on muscle in any of the specimens studied. Thus, it seems likely that increased NCAM expression in gastrointestinal smooth muscle in congenital aganglionosis is a result of the abnormal innervation rather than any symptomatic or clinical feature.
我们之前关于儿童先天性巨结肠症中神经细胞黏附分子（NCAM）的研究表明，在无神经节肠段的肠道肌肉，尤其是黏膜肌层中，NCAM表达增加。本研究旨在检验这种NCAM表达增加是先天性无神经节症的特征，还是便秘的非特异性特征。我们使用NCAM和蛋白基因产物9.5（PGP 9.5）的免疫细胞化学方法，研究了6例因特发性长期便秘接受手术治疗的患者（年龄20 - 60岁；5名女性和1名男性）的标本。结果显示，在成人便秘肠段中，NCAM的表达模式与对照组相似。在所研究的任何标本中，肌肉上均未检测到NCAM表达。因此，先天性无神经节症中胃肠道平滑肌NCAM表达增加似乎是异常神经支配的结果，而非任何症状或临床特征所致。
